Evaluation of cancer cell deformability by microcavity array

Tomoko Yoshino, Tsuyoshi Tanaka, Seita Nakamura, Ryo Negishi, Nozomi Shionoiri, Masahito Hosokawa, Tadashi Matsunaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalAnalytical Biochemistry
Volume520
DOIs
Publication statusPublished - 2017 Mar 1
Externally publishedYes

Keywords

  • Breast cancer
  • Cell deformability

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Yoshino, T., Tanaka, T., Nakamura, S., Negishi, R., Shionoiri, N., Hosokawa, M., & Matsunaga, T. (2017). Evaluation of cancer cell deformability by microcavity array. Analytical Biochemistry, 520, 16-21. https://doi.org/10.1016/j.ab.2016.12.026