Evaluation of cancer cell deformability by microcavity array

Tomoko Yoshino, Tsuyoshi Tanaka, Seita Nakamura, Ryo Negishi, Nozomi Shionoiri, Masahito Hosokawa, Tadashi Matsunaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalAnalytical Biochemistry
Volume520
DOIs
Publication statusPublished - 2017 Mar 1
Externally publishedYes

Fingerprint

Microcavities
Formability
Cells
Neoplasms
Confocal Microscopy
Pore size
Tumors
Microscopic examination
Breast
Throughput
Breast Neoplasms
Scanning
Pressure
Cell Line
Equipment and Supplies
Lasers

Keywords

  • Breast cancer
  • Cell deformability

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Yoshino, T., Tanaka, T., Nakamura, S., Negishi, R., Shionoiri, N., Hosokawa, M., & Matsunaga, T. (2017). Evaluation of cancer cell deformability by microcavity array. Analytical Biochemistry, 520, 16-21. https://doi.org/10.1016/j.ab.2016.12.026

Evaluation of cancer cell deformability by microcavity array. / Yoshino, Tomoko; Tanaka, Tsuyoshi; Nakamura, Seita; Negishi, Ryo; Shionoiri, Nozomi; Hosokawa, Masahito; Matsunaga, Tadashi.

In: Analytical Biochemistry, Vol. 520, 01.03.2017, p. 16-21.

Research output: Contribution to journalArticle

Yoshino, T, Tanaka, T, Nakamura, S, Negishi, R, Shionoiri, N, Hosokawa, M & Matsunaga, T 2017, 'Evaluation of cancer cell deformability by microcavity array', Analytical Biochemistry, vol. 520, pp. 16-21. https://doi.org/10.1016/j.ab.2016.12.026
Yoshino, Tomoko ; Tanaka, Tsuyoshi ; Nakamura, Seita ; Negishi, Ryo ; Shionoiri, Nozomi ; Hosokawa, Masahito ; Matsunaga, Tadashi. / Evaluation of cancer cell deformability by microcavity array. In: Analytical Biochemistry. 2017 ; Vol. 520. pp. 16-21.
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