Evaluation of cationic assemblies constructed with amino acid based lipids for plasmid DNA delivery

Yosuke Obata, Daisuke Suzuki, Shinji Takeoka

    Research output: Contribution to journalArticle

    65 Citations (Scopus)

    Abstract

    We synthesized cationic lipids bearing lysine, histidine, or arginine as a cationic headgroup for use in gene transfer studies. The cationic assemblies formed from lysine- or arginine-type lipids gave unilamellar vesicles (∼100 nm diameter), whereas the morphology of the histidine-type lipids was tube-like. The competences of the cationic assemblies were sufficient to form lipoplexes, and the resulting lipoplexes were evaluated in terms of gene expression efficiencies with COS-7 cells. The lysine- or arginine-type lipids exhibited higher gene expression efficiencies than that of Lipofectamine2000, a conventional transgenic reagent, indicating that stable lipoplexes could be prepared between spherical cationic assemblies and plasmid DNA. The gene expression efficiency in relation to the cationic headgroup of the lipids was as follows: lysine ≥ arginine ≥ histidine. In addition, gene expression efficiency was enhanced by decreasing the length of the alkyl chain of the hydrophobic moiety. Unlike Lipofectamine2000, no reduction in transfection efficiency in the presence of fetal bovine serum was observed for the lipoplexes formed using synthetic cationic lipids. Moreover, the synthetic cationic lipids revealed remarkably low cytotoxicity compared with Lipofectamine2000. In conclusion, cationic assemblies formed from 1,5-ditetradecyl-N-lysyl-L-glutamate or 1,5-ditetradecyl-N-arginyl-L-glutamate can be used as an effective plasmid DNA delivery system.

    Original languageEnglish
    Pages (from-to)1055-1063
    Number of pages9
    JournalBioconjugate Chemistry
    Volume19
    Issue number5
    DOIs
    Publication statusPublished - 2008 May

    Fingerprint

    Lipids
    Amino acids
    Plasmids
    DNA
    Arginine
    Amino Acids
    Gene expression
    Lysine
    Histidine
    Gene Expression
    Glutamic Acid
    Bearings (structural)
    Gene transfer
    Unilamellar Liposomes
    COS Cells
    Cytotoxicity
    Mental Competency
    Transfection
    Serum
    Genes

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    Cite this

    Evaluation of cationic assemblies constructed with amino acid based lipids for plasmid DNA delivery. / Obata, Yosuke; Suzuki, Daisuke; Takeoka, Shinji.

    In: Bioconjugate Chemistry, Vol. 19, No. 5, 05.2008, p. 1055-1063.

    Research output: Contribution to journalArticle

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