Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

Yosuke Obata, Gianni Ciofani, Vittoria Raffa, Alfred Cuschieri, Arianna Menciassi, Paolo Dario, Shinji Takeoka

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection. From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases.

Original languageEnglish
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume6
Issue number1
DOIs
Publication statusPublished - 2010 Feb
Externally publishedYes

Fingerprint

Liposomes
Lipids
Transfection
Amino acids
Green Fluorescent Proteins
Amino Acids
Cytotoxicity
Proteins
DNA
Nucleic acids
Cells
Nucleic Acids
Genes
Gene therapy
Glutamic Acid
Plasmids
Genetic Therapy
Cell Count
Cell Line
Lipofectamine

Keywords

  • Amino lipids
  • Cationic liposomes
  • Gene therapy
  • Neuronal transfection
  • Plasmid DNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. / Obata, Yosuke; Ciofani, Gianni; Raffa, Vittoria; Cuschieri, Alfred; Menciassi, Arianna; Dario, Paolo; Takeoka, Shinji.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 6, No. 1, 02.2010.

Research output: Contribution to journalArticle

Obata, Yosuke ; Ciofani, Gianni ; Raffa, Vittoria ; Cuschieri, Alfred ; Menciassi, Arianna ; Dario, Paolo ; Takeoka, Shinji. / Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2010 ; Vol. 6, No. 1.
@article{56e54f9854d84945936ef0c7493a33e8,
title = "Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection",
abstract = "We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16{\%} of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25{\%} by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection. From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases.",
keywords = "Amino lipids, Cationic liposomes, Gene therapy, Neuronal transfection, Plasmid DNA",
author = "Yosuke Obata and Gianni Ciofani and Vittoria Raffa and Alfred Cuschieri and Arianna Menciassi and Paolo Dario and Shinji Takeoka",
year = "2010",
month = "2",
doi = "10.1016/j.nano.2009.04.005",
language = "English",
volume = "6",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

AU - Obata, Yosuke

AU - Ciofani, Gianni

AU - Raffa, Vittoria

AU - Cuschieri, Alfred

AU - Menciassi, Arianna

AU - Dario, Paolo

AU - Takeoka, Shinji

PY - 2010/2

Y1 - 2010/2

N2 - We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection. From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases.

AB - We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C16) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C16 liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP-positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C16 lipoplexes, the proportion of GFP-positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C16, as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection. From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases.

KW - Amino lipids

KW - Cationic liposomes

KW - Gene therapy

KW - Neuronal transfection

KW - Plasmid DNA

UR - http://www.scopus.com/inward/record.url?scp=75349088429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75349088429&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2009.04.005

DO - 10.1016/j.nano.2009.04.005

M3 - Article

C2 - 19447207

AN - SCOPUS:75349088429

VL - 6

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 1

ER -