Evaluation of Enzyme Immunoassay using a Recombinant Envelope Protein Expressed in Insect Cells for Serological Confirmation of HTLV-I Infection

Kazuyo Yamashita, Midori Maekawa, Kohnosuke Mitani, Maki Wakamiya, Toshio Ogino, Kikuko Miyamura, Koichi Baba, Yoshiko Yamamoto, Hiroshi Nyunoya, Kunitada Shimotohno, Yutaka Takebe, Shudo Yamazaki

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1 Citation (Scopus)

Abstract

A recombinant human T-lymphotropic virus type I (HTLV-I) envelope protein expressed in insect cells using a recombinant baculovirus was employed as the antigen in an enzyme immunoassay (renvEIA). Peripheral blood samples were obtained from asymptomatic carriers or healthy individuals. Plasma was tested for HTLV-I antibody by renvEIA, particle agglutination, and Western immunoblot (WB), and lymphocyte DNA was tested for HTLV-I proviral DNA amplification by polymerase chain reaction (PCR). Of 61 people aged 9 months or older, 23 were positive (gag+, env+) and 19 others were in the “indeterminate” category (gag+, env-) when their WB results were interpreted according to the WHO-proposed criteria. Thirty-seven cases, including all of the WB+ cases and 14 of 19 WB indeterminate cases, were positive by renvEIA. In 34 of 37 renvEIA-positive cases, the presence of long terminal repeat (LTR) and tax/rex region of HTLV-I proviral DNA was detected by polymerase chain reaction (PCR) and following Southern blot hybridization. Thus, renvEIA would be a useful supplemental assay to confirm the presence of HTLV-I antibody in HTLV-I asymptomatic carriers.

Original languageEnglish
Pages (from-to)1857-1861
Number of pages5
JournalAIDS Research and Human Retroviruses
Volume8
Issue number11
DOIs
Publication statusPublished - 1992 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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  • Cite this

    Yamashita, K., Maekawa, M., Mitani, K., Wakamiya, M., Ogino, T., Miyamura, K., Baba, K., Yamamoto, Y., Nyunoya, H., Shimotohno, K., Takebe, Y., & Yamazaki, S. (1992). Evaluation of Enzyme Immunoassay using a Recombinant Envelope Protein Expressed in Insect Cells for Serological Confirmation of HTLV-I Infection. AIDS Research and Human Retroviruses, 8(11), 1857-1861. https://doi.org/10.1089/aid.1992.8.1857