Evaluation of the effect of high pressure carbon dioxide-pasteurized food on animal health

Ayane Hibi*, Tomoki Ohno, Akihiro Moriyama, Takehiro Himaki, Junko Takahashi, Hitoshi Iwahashi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


High pressure-carbon dioxide processing (HPCDP) is used as a non-thermal technology for food pasteurization to produce newer flavors for bread. However, there is no evidence that this process does not produce undesirable toxic materials. To confirm the safety of high pressure carbon dioxide-processed food, we fed high pressure carbon dioxide-processed bread to mice and evaluated their health by monitoring body weight, stress biomarkers, and gut microbiota. There was no significant difference in the body weight of normal and HPCDP bread-fed mice. Similarly, no significant difference in expression of genes important for maintaining the health of the mice was found. Although the mice’s gut microbiota showed a significant difference between the control and the two bread-fed groups, there was no significant difference between the HPCDP and the normal bread-fed groups. Therefore, we found no conclusive evidence to suggest that the processed bread contained toxic materials in comparison to the unprocessed bread.

Original languageEnglish
Pages (from-to)357-366
Number of pages10
JournalHigh Pressure Research
Issue number2
Publication statusPublished - 2019 Apr 3
Externally publishedYes


  • High pressure carbon dioxide
  • bread
  • microbiota

ASJC Scopus subject areas

  • Condensed Matter Physics


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