Evaluation of the properties of mechanically stretched poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane surfaces

Yoshikatsu Akiyama, Naoya Takeda, Masayuki Yamato, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Temperature-responsive cell culture surface (TRCS), poly(N-isopropylacrylamide) (PIPAAm) grafted tissue culture polystyrene (PIPAAm-TCPS), has been widely utilized as conventional TRCS in cell-sheet based tissue engineering filed. Recently, our group developed stretchable TRCS, which was PIPAAm gel grafted polydimethylsiloxane (PIPAAm-PDMS), to proof a concept that external mechanical stress could modulate the thickness of grafted PIPAAm layer as well as properties of PIPAAm-PDMS (Fig. 1). In the report, we demonstrated that external stretching and/or shrinking stress modulated cell attachment and detachment behavior, probably because of altering the degree of dehydration and hydration of grafted PIPAAm chains. In this study, we prepared PIPAAm-PDMSs with large and low density of grafted PIPAAm. In order to further investigate the mechanical stress modulated cell attachment and detachment properties, properties (the amount of adsorbed fibronectin (FN), wettability, and cell attachment) of these two PIPAAm-PDMS surfaces at stretched and/or stretched states were evaluated and compared.

Original languageEnglish
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
Publication statusPublished - 2019 Jan 1
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: 2019 Apr 32019 Apr 6

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period19/4/319/4/6

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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  • Cite this

    Akiyama, Y., Takeda, N., Yamato, M., & Okano, T. (2019). Evaluation of the properties of mechanically stretched poly(N-isopropylacrylamide) gel grafted polydimethylsiloxane surfaces. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.