Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations

Yukako Koike, Akiko Yoneyama, Jun Shirai, Tateru Ishida, Eriko Shoda, Kouji Miyazaki, Sinji Sunaga, Ryoichi Horie, Katunori Aoki, Kazuhiko Koike, Iturou Ogata, Tomoyuki Tahara, Takashi Kato, Kazuhiko Nakahara, Toshitugu Kariya, Masaaki Higashihara

Research output: Contribution to journalArticle

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Abstract

To evaluate thrombopoiesis in thrombocytopenic disorders, we simultaneously determined reticulated platelet counts in whole blood by FACScan flow cytometry and serum thrombopoietin (TPO) concentrations by a sensitive sandwich ELISA. The subjects were 40 healthy volunteers and 45 thrombocytopenic patients. In idiopathic thrombocytopenic purpura (ITP), the percentage of reticulated platelets was significantly elevated (5.61 ± 2.02%: mean ± SD) relative to normal controls (2.17 ± 0.90%), but serum TPO concentrations (1.91 ± 1.27 fmol/l) did not differ significantly from the normal range (1.43 ± 0.62 fmol/l). The patients with aplastic anemia (AA) had decreased reticulated platelet counts and markedly increased serum TPO concentrations (13.65 ± 10.64 fmol/l). In thrombocytopenic patients with liver cirrhosis (LC), the absolute number of reticulated platelets (1.65 ± 1.11 x 109/l) decreased similarly that in AA. However, serum TPO concentrations (1.38 ± 0.50 fmol/l) did not increase in contrast to AA. Our findings suggested a possible dual mechanism of thrombocytopenia in LC; that is, thrombocytopenia in LC results from the decreased TPO production primarily in the liver adding to an increase in platelet sequestration in the spleen.

Original languageEnglish
Pages (from-to)1106-1110
Number of pages5
JournalThrombosis and Haemostasis
Volume79
Issue number6
Publication statusPublished - 1998
Externally publishedYes

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Thrombopoiesis
Thrombopoietin
Blood Platelets
Aplastic Anemia
Platelet Count
Liver Cirrhosis
Serum
Thrombocytopenia
Idiopathic Thrombocytopenic Purpura
Healthy Volunteers
Flow Cytometry
Reference Values
Spleen
Enzyme-Linked Immunosorbent Assay
Liver

ASJC Scopus subject areas

  • Hematology

Cite this

Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations. / Koike, Yukako; Yoneyama, Akiko; Shirai, Jun; Ishida, Tateru; Shoda, Eriko; Miyazaki, Kouji; Sunaga, Sinji; Horie, Ryoichi; Aoki, Katunori; Koike, Kazuhiko; Ogata, Iturou; Tahara, Tomoyuki; Kato, Takashi; Nakahara, Kazuhiko; Kariya, Toshitugu; Higashihara, Masaaki.

In: Thrombosis and Haemostasis, Vol. 79, No. 6, 1998, p. 1106-1110.

Research output: Contribution to journalArticle

Koike, Y, Yoneyama, A, Shirai, J, Ishida, T, Shoda, E, Miyazaki, K, Sunaga, S, Horie, R, Aoki, K, Koike, K, Ogata, I, Tahara, T, Kato, T, Nakahara, K, Kariya, T & Higashihara, M 1998, 'Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations', Thrombosis and Haemostasis, vol. 79, no. 6, pp. 1106-1110.
Koike, Yukako ; Yoneyama, Akiko ; Shirai, Jun ; Ishida, Tateru ; Shoda, Eriko ; Miyazaki, Kouji ; Sunaga, Sinji ; Horie, Ryoichi ; Aoki, Katunori ; Koike, Kazuhiko ; Ogata, Iturou ; Tahara, Tomoyuki ; Kato, Takashi ; Nakahara, Kazuhiko ; Kariya, Toshitugu ; Higashihara, Masaaki. / Evaluation of thrombopoiesis in thrombocytopenic disorders by simultaneous measurement of reticulated platelets of whole blood and serum thrombopoietin concentrations. In: Thrombosis and Haemostasis. 1998 ; Vol. 79, No. 6. pp. 1106-1110.
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AU - Shoda, Eriko

AU - Miyazaki, Kouji

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AU - Horie, Ryoichi

AU - Aoki, Katunori

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AU - Tahara, Tomoyuki

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