Evidence for the involvement of inositol trisphosphate but not cyclic nucleotides in visual transduction in Hermissenda eye

Manabu Sakakibara, Hiroko Inoue, Tohru Yoshioka

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Although several second messengers are known to be involved in invertebrate photoresponses, the mechanism underlying invertebrate phototransduction remains unclear. In the present study, brief injection of inositol trisphosphate into Hermissenda photoreceptors induced a transient Na+ current followed by burst activity, which accurately reproduced the native photoresponse. Injection of Ca2+ did not induce a significant change in the membrane potential but potentiated the : native photoresponse. Injection of a Ca2+ chelator decreased the response amplitude and increased the response latency. Injection of cGMP induced a Ca2+-dependent, transient depolarization with a short latency. cAMP injection evoked Na+-dependent action potentials without a rise in membrane potential. Taken together, these results suggest that phototransduction in Hermissenda is mediated by Na+ channels that are directly activated by inositol trisphosphate without mobilization of cytosolic Ca2+.

    Original languageEnglish
    Pages (from-to)20795-20801
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume273
    Issue number33
    DOIs
    Publication statusPublished - 1998 Aug 14

    Fingerprint

    Hermissenda
    Cyclic Nucleotides
    Inositol
    Membranes
    Injections
    Light Signal Transduction
    Depolarization
    Second Messenger Systems
    Chelating Agents
    Invertebrates
    Membrane Potentials
    Action Potentials
    Reaction Time

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Evidence for the involvement of inositol trisphosphate but not cyclic nucleotides in visual transduction in Hermissenda eye. / Sakakibara, Manabu; Inoue, Hiroko; Yoshioka, Tohru.

    In: Journal of Biological Chemistry, Vol. 273, No. 33, 14.08.1998, p. 20795-20801.

    Research output: Contribution to journalArticle

    Sakakibara, Manabu ; Inoue, Hiroko ; Yoshioka, Tohru. / Evidence for the involvement of inositol trisphosphate but not cyclic nucleotides in visual transduction in Hermissenda eye. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 33. pp. 20795-20801.
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