Evolutionary origin of GnIH and NPFF in chordates

Insights from novel amphioxus RFamide peptides

Tomohiro Osugi, Tomoki Okamura, You Lee Son, Makoto Ohkubo, Takayoshi Ubuka, Yasuhisa Henmi, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Gonadotropin-inhibitory hormone (GnIH) is a newly identified hypothalamic neuropeptide that inhibits pituitary hormone secretion in vertebrates. GnIH has an LPXRFamide (X = L or Q) motif at the C-terminal in representative species of gnathostomes. On the other hand, neuropeptide FF (NPFF), a neuropeptide characterized as a pain-modulatory neuropeptide, in vertebrates has a PQRFamide motif similar to the C-terminal of GnIH, suggesting that GnIH and NPFF have diverged from a common ancestor. Because GnIH and NPFF belong to the RFamide peptide family in vertebrates, protochordate RFamide peptides may provide important insights into the evolutionary origin of GnIH and NPFF. In this study, we identified a novel gene encoding RFamide peptides and two genes of their putative receptors in the amphioxus Branchiostoma japonicum. Molecular phylogenetic analysis and synteny analysis indicated that these genes are closely related to the genes of GnIH and NPFF and their receptors of vertebrates. We further identified mature RFamide peptides and their receptors in protochordates. The identified amphioxus RFamide peptides inhibited forskolin induced cAMP signaling in the COS-7 cells with one of the identified amphioxus RFamide peptide receptors expressed. These results indicate that the identified protochordate RFamide peptide gene is a common ancestral form of GnIH and NPFF genes, suggesting that the origin of GnIH and NPFF may date back to the time of the emergence of early chordates. GnIH gene and NPFF gene may have diverged by whole-genome duplication in the course of vertebrate evolution.

    Original languageEnglish
    Article numbere100962
    JournalPLoS One
    Volume9
    Issue number7
    DOIs
    Publication statusPublished - 2014 Jul 1

    Fingerprint

    Lancelets
    Chordata
    neuropeptides
    gonadotropins
    Gonadotropins
    hormones
    Hormones
    peptides
    Genes
    Vertebrates
    vertebrates
    Neuropeptides
    genes
    Peptide Receptors
    receptors
    phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide
    RFamide peptide
    Synteny
    Gene encoding
    pituitary hormones

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Evolutionary origin of GnIH and NPFF in chordates : Insights from novel amphioxus RFamide peptides. / Osugi, Tomohiro; Okamura, Tomoki; Son, You Lee; Ohkubo, Makoto; Ubuka, Takayoshi; Henmi, Yasuhisa; Tsutsui, Kazuyoshi.

    In: PLoS One, Vol. 9, No. 7, e100962, 01.07.2014.

    Research output: Contribution to journalArticle

    Osugi, Tomohiro ; Okamura, Tomoki ; Son, You Lee ; Ohkubo, Makoto ; Ubuka, Takayoshi ; Henmi, Yasuhisa ; Tsutsui, Kazuyoshi. / Evolutionary origin of GnIH and NPFF in chordates : Insights from novel amphioxus RFamide peptides. In: PLoS One. 2014 ; Vol. 9, No. 7.
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    AU - Ubuka, Takayoshi

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