Exercise-induced oxidative DNA damage and lymphocytopenia in sedentary young males

Yuko Tanimura, Kazuhiro Shimizu, Kai Tanabe, Takeshi Otsuki, Ryohei Yamauchi, Yuichi Matsubara, Motoyuki Iemitsu, Seiji Maeda, Ryuichi Ajisaka

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Post high-intensity exercise lymphocytopenia is well documented, but its underlying mechanisms have not been fully elucidated. A possible mechanism is a reactive oxygen species-induced DNA damage after high-intensity exercise. Furthermore, lymphocyte apoptosis related to DNA damage might contribute to exercise-induced lymphocytopenia. Purpose: This study examined lymphocytopenia, lymphocyte oxidative DNA damage, and apoptosis in young healthy sedentary males after acute high-intensity exercise. Method: Fifteen subjects exercised on bicycle ergometers for 1 h at 75% of their V̇O2max. Venous blood samples were taken before exercise (PRE) and hourly after exercise until 4 h (P0-P4). Lymphocyte counts, oxidative DNA damage evaluated using the Comet assay with human 8-oxoguanine DNA glycosylase, and serum lipid peroxide (LPO) concentration were measured. Furthermore, lymphocyte superoxide, Fas receptor (CD95), and Annexin-V-positive lymphocyte apoptosis cells were measured in 10 subjects who exercised and gave blood samples as described above. Results: Lymphocyte counts became significantly lower than the PRE value (P < 0.05): 20.4% at P1, 24.3% at P2, and 16.3% at P3. Moreover, LPO significantly increased by P2 (P < 0.05): 1.6-fold. The % DNA in tail, indicating oxidative DNA damage, was significantly higher at P3 (54.3 ± 12.8%) than at PRE (42.6 ± 11.1%, P < 0.05). The lymphocyte superoxide level was significantly higher (51.3%) than the PRE value (P < 0.05). Neither CD95 nor Annexin-V-positive cells were significantly different than the PRE value. Conclusion: Results of this study suggest that lymphocyte oxidative DNA damage can relate to lymphocytopenia, although DNA damage was not associated with apoptosis in healthy young sedentary males.

Original languageEnglish
Pages (from-to)1455-1462
Number of pages8
JournalMedicine and Science in Sports and Exercise
Volume40
Issue number8
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Annexin
  • Comet assay
  • FAS receptor
  • High-intensity exercise
  • hOGG1 enzyme
  • Reactive oxygen species

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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