Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium

Zsolt Radak, Mária Sasvári, Csaba Nyakas, Jozsef Pucsok, Hideko Nakamoto, Sataro Goto

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Both regular physical exercise and low levels of H2O2 administration result in increased resistance to oxidative stress. We measured the accumulation of reactive carbonyl derivatives and the activities of proteasome complex and DT-diaphorase in cardiac muscle of trained and untrained rats after chronic i.p. administration of 1 ml t-butyl H2O2 (1 mmol/kg for 3 weeks every second day). Twenty-four rats were randomly assigned to a control group administered with saline, control administered with H2O2, and exercised administered either saline or H2O2. The activity of DT-diaphorase significantly increased in H2O2 administered and exercised groups, indicating that an increase in H2O2 levels stimulate the activity of this enzyme. The cardiac muscle of H2O2 administered nonexercised animals accumulated significantly more carbonyl than control group (P < 0.05). The exercise and H2O2 administration resulted in less oxidatively modified protein than found in nonexercised groups (P < 0.05). The peptide- like activity of proteasome complex was induced by the treatment of H2O2 and exercise and exercise potentiate the effect of H2O2. On the other hand, the chymotrypsin-like and trypsin-like activities were stimulated only by physical training and H2O2 administration. The data suggest that chronic administration of H2O2 after exercise training decreases the accumulation of carbonyl groups below the steady-state level and induces the activity of proteasome and DT-diaphorase. Hence, the stimulating effect of physical exercise on free radical generation is an important phenomenon of the exercise-induced adaptation process since it increases resistance to oxidative stress. Regular exercise training is a valuable physiological means of preconditioning the myocardium to prolonged oxidative stress. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume376
Issue number2
DOIs
Publication statusPublished - 2000 Apr 15
Externally publishedYes

Fingerprint

NAD(P)H Dehydrogenase (Quinone)
Hydrogen Peroxide
Rats
Myocardium
Proteasome Endopeptidase Complex
Oxidative stress
Exercise
Oxidative Stress
Proteins
Muscle
Control Groups
Free Radicals
Chymotrypsin
Trypsin
Animals
Peptides
Derivatives
Enzymes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. / Radak, Zsolt; Sasvári, Mária; Nyakas, Csaba; Pucsok, Jozsef; Nakamoto, Hideko; Goto, Sataro.

In: Archives of Biochemistry and Biophysics, Vol. 376, No. 2, 15.04.2000, p. 248-251.

Research output: Contribution to journalArticle

Radak, Zsolt ; Sasvári, Mária ; Nyakas, Csaba ; Pucsok, Jozsef ; Nakamoto, Hideko ; Goto, Sataro. / Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium. In: Archives of Biochemistry and Biophysics. 2000 ; Vol. 376, No. 2. pp. 248-251.
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