Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice

Noriaki Kawanishi, Hiroyuki Niihara, Tsubasa Mizokami, Hiromi Yano, Katsuhiko Suzuki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Tissue fibrosis, such as that which occurs in obesity, is associated with chronic inflammatory diseases. Although regular exercise reduces adipose tissue inflammation, the mechanisms regulating the effects of exercise on adipose tissue fibrosis are unclear. This study aimed to clarify whether exercise training attenuates adipose tissue fibrosis with consequent reduction of extracellular matrix including collagens. Male C57BL/6J (4-week old) mice were randomly assigned to four groups that received a normal diet (ND) plus sedentary (n = 8), an ND plus exercise training (n = 8), a high-fat diet (HFD) plus sedentary (n = 12), and an HFD plus exercise training (n = 12). Mice were fed the ND or HFD from 4 to 20 weeks of age. The exercise groups were trained on a motorized treadmill for 60 min/day, 5 times/week over the same period. Histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining were attenuated in HFD exercise mice compared with HFD sedentary mice. mRNA levels of transforming growth factor-β and tissue inhibitors of metalloproteinase-1, major regulators of tissue fibrosis, were increased in HFD sedentary mice but were attenuated in HFD exercise mice. Similarly, adipose tissue from the HFD sedentary mice contained higher macrophages than adipose tissue from the ND mice, and this was also lowered by exercise training. These findings suggest that exercise training may be effective for attenuating adipose tissue inflammation in obesity.

Original languageEnglish
Pages (from-to)774-779
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume440
Issue number4
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

Obese Mice
High Fat Diet
Nutrition
Adipose Tissue
Fibrosis
Exercise
Tissue
Diet
Fats
Obesity
Inflammation
Tissue Inhibitor of Metalloproteinase-1
Transforming Growth Factors
Extracellular Matrix
Smooth Muscle
Actins
Exercise equipment
Chronic Disease
Collagen
Macrophages

Keywords

  • Collagen
  • High-fat diet
  • Macrophage
  • TGF-β
  • TIMP-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice. / Kawanishi, Noriaki; Niihara, Hiroyuki; Mizokami, Tsubasa; Yano, Hiromi; Suzuki, Katsuhiko.

In: Biochemical and Biophysical Research Communications, Vol. 440, No. 4, 01.11.2013, p. 774-779.

Research output: Contribution to journalArticle

Kawanishi, Noriaki ; Niihara, Hiroyuki ; Mizokami, Tsubasa ; Yano, Hiromi ; Suzuki, Katsuhiko. / Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 440, No. 4. pp. 774-779.
@article{7183f6675ade4378b9a065ff864e5881,
title = "Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice",
abstract = "Tissue fibrosis, such as that which occurs in obesity, is associated with chronic inflammatory diseases. Although regular exercise reduces adipose tissue inflammation, the mechanisms regulating the effects of exercise on adipose tissue fibrosis are unclear. This study aimed to clarify whether exercise training attenuates adipose tissue fibrosis with consequent reduction of extracellular matrix including collagens. Male C57BL/6J (4-week old) mice were randomly assigned to four groups that received a normal diet (ND) plus sedentary (n = 8), an ND plus exercise training (n = 8), a high-fat diet (HFD) plus sedentary (n = 12), and an HFD plus exercise training (n = 12). Mice were fed the ND or HFD from 4 to 20 weeks of age. The exercise groups were trained on a motorized treadmill for 60 min/day, 5 times/week over the same period. Histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining were attenuated in HFD exercise mice compared with HFD sedentary mice. mRNA levels of transforming growth factor-β and tissue inhibitors of metalloproteinase-1, major regulators of tissue fibrosis, were increased in HFD sedentary mice but were attenuated in HFD exercise mice. Similarly, adipose tissue from the HFD sedentary mice contained higher macrophages than adipose tissue from the ND mice, and this was also lowered by exercise training. These findings suggest that exercise training may be effective for attenuating adipose tissue inflammation in obesity.",
keywords = "Collagen, High-fat diet, Macrophage, TGF-β, TIMP-1",
author = "Noriaki Kawanishi and Hiroyuki Niihara and Tsubasa Mizokami and Hiromi Yano and Katsuhiko Suzuki",
year = "2013",
month = "11",
day = "1",
doi = "10.1016/j.bbrc.2013.10.004",
language = "English",
volume = "440",
pages = "774--779",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice

AU - Kawanishi, Noriaki

AU - Niihara, Hiroyuki

AU - Mizokami, Tsubasa

AU - Yano, Hiromi

AU - Suzuki, Katsuhiko

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Tissue fibrosis, such as that which occurs in obesity, is associated with chronic inflammatory diseases. Although regular exercise reduces adipose tissue inflammation, the mechanisms regulating the effects of exercise on adipose tissue fibrosis are unclear. This study aimed to clarify whether exercise training attenuates adipose tissue fibrosis with consequent reduction of extracellular matrix including collagens. Male C57BL/6J (4-week old) mice were randomly assigned to four groups that received a normal diet (ND) plus sedentary (n = 8), an ND plus exercise training (n = 8), a high-fat diet (HFD) plus sedentary (n = 12), and an HFD plus exercise training (n = 12). Mice were fed the ND or HFD from 4 to 20 weeks of age. The exercise groups were trained on a motorized treadmill for 60 min/day, 5 times/week over the same period. Histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining were attenuated in HFD exercise mice compared with HFD sedentary mice. mRNA levels of transforming growth factor-β and tissue inhibitors of metalloproteinase-1, major regulators of tissue fibrosis, were increased in HFD sedentary mice but were attenuated in HFD exercise mice. Similarly, adipose tissue from the HFD sedentary mice contained higher macrophages than adipose tissue from the ND mice, and this was also lowered by exercise training. These findings suggest that exercise training may be effective for attenuating adipose tissue inflammation in obesity.

AB - Tissue fibrosis, such as that which occurs in obesity, is associated with chronic inflammatory diseases. Although regular exercise reduces adipose tissue inflammation, the mechanisms regulating the effects of exercise on adipose tissue fibrosis are unclear. This study aimed to clarify whether exercise training attenuates adipose tissue fibrosis with consequent reduction of extracellular matrix including collagens. Male C57BL/6J (4-week old) mice were randomly assigned to four groups that received a normal diet (ND) plus sedentary (n = 8), an ND plus exercise training (n = 8), a high-fat diet (HFD) plus sedentary (n = 12), and an HFD plus exercise training (n = 12). Mice were fed the ND or HFD from 4 to 20 weeks of age. The exercise groups were trained on a motorized treadmill for 60 min/day, 5 times/week over the same period. Histological hepatic fibrosis detected by Sirius red and α-smooth muscle actin staining were attenuated in HFD exercise mice compared with HFD sedentary mice. mRNA levels of transforming growth factor-β and tissue inhibitors of metalloproteinase-1, major regulators of tissue fibrosis, were increased in HFD sedentary mice but were attenuated in HFD exercise mice. Similarly, adipose tissue from the HFD sedentary mice contained higher macrophages than adipose tissue from the ND mice, and this was also lowered by exercise training. These findings suggest that exercise training may be effective for attenuating adipose tissue inflammation in obesity.

KW - Collagen

KW - High-fat diet

KW - Macrophage

KW - TGF-β

KW - TIMP-1

UR - http://www.scopus.com/inward/record.url?scp=84887055766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887055766&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2013.10.004

DO - 10.1016/j.bbrc.2013.10.004

M3 - Article

VL - 440

SP - 774

EP - 779

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -