Exercise training decreases DNA damage and increases DNA repair and resistance against oxidative stress of proteins in aged rat skeletal muscle

Zsolt Radak, Hisashi Naito, Takao Kaneko, Shunichi Tahara, Hideko Nakamoto, Ryoya Takahashi, Fernando Cardozo-Pelaez, Sataro Goto

Research output: Contribution to journalArticle

176 Citations (Scopus)


Regular physical exercise retards a number of age-associated disorders, in spite of the paradox that free radical generation is significantly enhanced with exercise. Eight weeks of treadmill running resulted in nearly a 40% increase in maximal oxygen uptake in both middle-aged (20-month-old) and aged (30-month-old) rats. The age-associated increase in 8-hydroxy-2′-deoxyguanosine (8-OHdG) content was significantly attenuated in gastrocnemius muscle by exercise. The 8-OHdG repair, as measured by the excision of 32P-labeled damaged oligonucleotide, increased in muscle of exercising animals. The reactive carbonyl derivatives (RCD) of proteins did not increase with aging. However, when the muscle homogenate was exposed to a mixture of 1 mM iron sulfate and 50 mM ascorbic acid, the muscle of old control animals accumulated more RCD than that of the trained or adult groups. The chymotrypsin-like activity of proteasome complex increased in muscle of old trained rats. We suggest that regular exercise-induced adaptation attenuates the age-associated increase in 8-OHdG levels, and increases the activity of DNA repair and resistance against oxidative stress in proteins.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Issue number2
Publication statusPublished - 2002 Jan 1
Externally publishedYes



  • Aging
  • Base excision repair
  • DNA repair
  • Exercise
  • Oxidative stress
  • Proteasome

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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