Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF

Yoko Azumaya, Shiro Kominami, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

From brains of the adult male zebra finch, we purified a peptide that has a homologous sequence of the large subunit of human U2AF. U2AF is a non-small-nuclear ribonucleoprotein (snRNP) splicing factor required for pre-spliceosome assembly. U2AF consists of a large and a small subunit, whereas only a large subunit is required for in vitro splicing. To show that U2AF large-subunit-like protein exists in the brain of zebra finches, we conducted immunoblot analysis on brain extract, using antiserum against an isolated peptide with primary structures similar to the U2AF large subunit (ppU2AF1s). The immunoblot analysis showed that a protein with a molecular weight of about 60 kd reacted with the anti-ppU2AF1s-antibody to a putative peptide of the U2AF large subunit. To examine the localization of this protein in the brain, we also conducted immunohistochemical analysis with the anti-peptide. An intense immunoreaction was restricted within the cellular nucleus throughout the brain, suggesting that this protein may contribute to the pre-spliceosome assembly in almost all of the regions of the brain.

Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalJournal of Experimental Zoology
Volume281
Issue number2
DOIs
Publication statusPublished - 1998 Jun 1
Externally publishedYes

Fingerprint

Taeniopygia guttata
brain
spliceosomes
peptides
proteins
ribonucleoproteins
sequence homology
antiserum
molecular weight
antibodies
extracts

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF. / Azumaya, Yoko; Kominami, Shiro; Tsutsui, Kazuyoshi.

In: Journal of Experimental Zoology, Vol. 281, No. 2, 01.06.1998, p. 90-96.

Research output: Contribution to journalArticle

@article{b2cd7d97dadc42f39c7280e6209cac7a,
title = "Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF",
abstract = "From brains of the adult male zebra finch, we purified a peptide that has a homologous sequence of the large subunit of human U2AF. U2AF is a non-small-nuclear ribonucleoprotein (snRNP) splicing factor required for pre-spliceosome assembly. U2AF consists of a large and a small subunit, whereas only a large subunit is required for in vitro splicing. To show that U2AF large-subunit-like protein exists in the brain of zebra finches, we conducted immunoblot analysis on brain extract, using antiserum against an isolated peptide with primary structures similar to the U2AF large subunit (ppU2AF1s). The immunoblot analysis showed that a protein with a molecular weight of about 60 kd reacted with the anti-ppU2AF1s-antibody to a putative peptide of the U2AF large subunit. To examine the localization of this protein in the brain, we also conducted immunohistochemical analysis with the anti-peptide. An intense immunoreaction was restricted within the cellular nucleus throughout the brain, suggesting that this protein may contribute to the pre-spliceosome assembly in almost all of the regions of the brain.",
author = "Yoko Azumaya and Shiro Kominami and Kazuyoshi Tsutsui",
year = "1998",
month = "6",
day = "1",
doi = "10.1002/(SICI)1097-010X(19980601)281:2<90::AID-JEZ3>3.0.CO;2-5",
language = "English",
volume = "281",
pages = "90--96",
journal = "Journal of Experimental Zoology",
issn = "0022-104X",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Existence and localization of a protein in zebra finch brain with similar structural features as the large subunit of the splicing factor U2AF

AU - Azumaya, Yoko

AU - Kominami, Shiro

AU - Tsutsui, Kazuyoshi

PY - 1998/6/1

Y1 - 1998/6/1

N2 - From brains of the adult male zebra finch, we purified a peptide that has a homologous sequence of the large subunit of human U2AF. U2AF is a non-small-nuclear ribonucleoprotein (snRNP) splicing factor required for pre-spliceosome assembly. U2AF consists of a large and a small subunit, whereas only a large subunit is required for in vitro splicing. To show that U2AF large-subunit-like protein exists in the brain of zebra finches, we conducted immunoblot analysis on brain extract, using antiserum against an isolated peptide with primary structures similar to the U2AF large subunit (ppU2AF1s). The immunoblot analysis showed that a protein with a molecular weight of about 60 kd reacted with the anti-ppU2AF1s-antibody to a putative peptide of the U2AF large subunit. To examine the localization of this protein in the brain, we also conducted immunohistochemical analysis with the anti-peptide. An intense immunoreaction was restricted within the cellular nucleus throughout the brain, suggesting that this protein may contribute to the pre-spliceosome assembly in almost all of the regions of the brain.

AB - From brains of the adult male zebra finch, we purified a peptide that has a homologous sequence of the large subunit of human U2AF. U2AF is a non-small-nuclear ribonucleoprotein (snRNP) splicing factor required for pre-spliceosome assembly. U2AF consists of a large and a small subunit, whereas only a large subunit is required for in vitro splicing. To show that U2AF large-subunit-like protein exists in the brain of zebra finches, we conducted immunoblot analysis on brain extract, using antiserum against an isolated peptide with primary structures similar to the U2AF large subunit (ppU2AF1s). The immunoblot analysis showed that a protein with a molecular weight of about 60 kd reacted with the anti-ppU2AF1s-antibody to a putative peptide of the U2AF large subunit. To examine the localization of this protein in the brain, we also conducted immunohistochemical analysis with the anti-peptide. An intense immunoreaction was restricted within the cellular nucleus throughout the brain, suggesting that this protein may contribute to the pre-spliceosome assembly in almost all of the regions of the brain.

UR - http://www.scopus.com/inward/record.url?scp=0032104840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032104840&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-010X(19980601)281:2<90::AID-JEZ3>3.0.CO;2-5

DO - 10.1002/(SICI)1097-010X(19980601)281:2<90::AID-JEZ3>3.0.CO;2-5

M3 - Article

VL - 281

SP - 90

EP - 96

JO - Journal of Experimental Zoology

JF - Journal of Experimental Zoology

SN - 0022-104X

IS - 2

ER -