Existence of a photoinducible phase for ovarian development and photoperiod-related alteration of clock gene expression in a damselfish

Yuki Takeuchi, Noriko Hada, Satoshi Imamura, Sung Pyo Hur, Selma Bouchekioua, Akihiro Takemura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The sapphire devil, Chrysiptera cyanea, is a reef-associated damselfish and their ovarian development can be induced by a long photoperiod. In this study, we demonstrated the existence of a photoinducible phase for the photoperiodic ovarian development in the sapphire devil. Induction of ovarian development under night-interruption light schedules and Nanda-Hamner cycles revealed that the photoinducible phase appeared in a circadian manner between ZT12 and ZT13. To characterize the effect of photoperiod on clock gene expression in the brain of this species, we determined the expression levels of the sdPer1, sdPer2, sdCry1, and sdCry2 clock genes under constant light and dark conditions (LL and DD) and photoperiodic (short and long photoperiods). The expression of sdPer1 exhibited clear circadian oscillation under both LL and DD conditions, while sdPer2 and sdCry1 expression levels were lower under DD than under LL conditions and sdCry2 expression was lower under LL than under DD conditions. These results suggest a key role for sdPer1 in circadian clock cycling and that sdPer2, sdCry1, and sdCry2 are light-responsive clock genes in the sapphire devil. After 1. week under a long photoperiod, we observed photoperiod-related changes in sdPer1, sdPer2, and sdCry2 expression, but not in sdCry1 expression. These results suggest that the expression patterns of some clock genes exhibit seasonal variation according to seasonal changes in day length and that such seasonal alteration of clock gene expression may contribute to seasonal recognition by the sapphire devil.

Original languageEnglish
Pages (from-to)32-39
Number of pages8
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume188
DOIs
Publication statusPublished - 2015 Oct 1
Externally publishedYes

Fingerprint

Photoperiod
Gene expression
Aluminum Oxide
Clocks
Gene Expression
Light
Genes
Circadian Clocks
Reefs
Appointments and Schedules
Brain

Keywords

  • Circadian clock
  • Cry1
  • Cry2
  • Per1
  • Per2
  • Photoperiodism
  • Seasonality

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Existence of a photoinducible phase for ovarian development and photoperiod-related alteration of clock gene expression in a damselfish. / Takeuchi, Yuki; Hada, Noriko; Imamura, Satoshi; Hur, Sung Pyo; Bouchekioua, Selma; Takemura, Akihiro.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 188, 01.10.2015, p. 32-39.

Research output: Contribution to journalArticle

Takeuchi, Yuki ; Hada, Noriko ; Imamura, Satoshi ; Hur, Sung Pyo ; Bouchekioua, Selma ; Takemura, Akihiro. / Existence of a photoinducible phase for ovarian development and photoperiod-related alteration of clock gene expression in a damselfish. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2015 ; Vol. 188. pp. 32-39.
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