Existence of nuclear-encoded 5S-rRNA in bovine mitochondria

Shigeo Yoshionari, Tomohiro Koike, Takashi Yokogawa, Kazuya Nishikawa, Takuya Ueda, Kinichiro Miura, Kimitsuna Watanabe

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A number of proteins functioning in mitochondria are synthesized in the cytoplasm and imported into the mitochondria via specific transport systems. In mammals, on the contrary, mitochondrial membranes have generally been considered to be impermeable to nucleic acids. However, here we show that an RNA with 120 nucleotides, the sequence of which is identical to that of the nuclear-encoded 5S RNA, exists in bovine mitochondria, although the mitochondrial genome encodes no 5S RNA gene. This RNA molecule was found to be retained in purified bovine mitochondria as well as in the mitoplasts, even after extensive treatment with an RNase, demonstrating that the 5S RNA is actually located inside the mitochondrial inner membrane. The 5S rRNA molecule was also shown to exist in mitochondria from rabbit and chicken.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalFEBS Letters
Volume338
Issue number2
DOIs
Publication statusPublished - 1994 Jan 31
Externally publishedYes

Keywords

  • 5S-rRNA
  • Bovine
  • Mitochondria
  • Transport

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Yoshionari, S., Koike, T., Yokogawa, T., Nishikawa, K., Ueda, T., Miura, K., & Watanabe, K. (1994). Existence of nuclear-encoded 5S-rRNA in bovine mitochondria. FEBS Letters, 338(2), 137-142. https://doi.org/10.1016/0014-5793(94)80351-X