Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses

Yo Kikuchi, Yumiko Ando, Nobuko Ichimura, Akihiro Noda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Highly purified and commercially available preparations of reverse transcriptases from retroviruses contain a 3′ to 5′ exoribonuclease activity capable of hydrolyzing synthetic homopolyribonucleotides having a 3′ -OH end. The exoribonuclease activity of reverse transcriptase preparations from Rous associated virus-2 was further characterized. This exoribonuclease activity cleaves poly(C) and poly(U) exonucleolytically from the 3′-OH end to produce nucleoside 5′-phosphates. Poly(A), poly(G), circular polyribonucleotide, and double-stranded polyribonucleotide were not hydrolyzed by the activity. This is a novel type of exoribonuclease activity.

Original languageEnglish
Pages (from-to)974-978
Number of pages5
JournalJournal of Biochemistry
Volume105
Issue number6
Publication statusPublished - 1989 Jun
Externally publishedYes

Fingerprint

Exoribonucleases
RNA-Directed DNA Polymerase
Retroviridae
Polyribonucleotides
Viruses
Reverse
Preparation
Phosphates
Poly G
Avian Leukosis Virus
Poly U
Poly A
Nucleosides
Phosphate
Virus

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Kikuchi, Y., Ando, Y., Ichimura, N., & Noda, A. (1989). Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses. Journal of Biochemistry, 105(6), 974-978.

Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses. / Kikuchi, Yo; Ando, Yumiko; Ichimura, Nobuko; Noda, Akihiro.

In: Journal of Biochemistry, Vol. 105, No. 6, 06.1989, p. 974-978.

Research output: Contribution to journalArticle

Kikuchi, Y, Ando, Y, Ichimura, N & Noda, A 1989, 'Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses', Journal of Biochemistry, vol. 105, no. 6, pp. 974-978.
Kikuchi, Yo ; Ando, Yumiko ; Ichimura, Nobuko ; Noda, Akihiro. / Exoribonuclease activity of purified reverse transcriptase preparations from retroviruses. In: Journal of Biochemistry. 1989 ; Vol. 105, No. 6. pp. 974-978.
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