Experimental and simulation analysis of community structure of nitrifying bacteria in a membrane-aerated biofilm

S. Matsumoto, A. Terada, Y. Aoi, S. Tsuneda, E. Alpkvist, C. Picioreanu, M. C.M. van Loosdrecht

Research output: Chapter in Book/Report/Conference proceedingConference contribution

37 Citations (Scopus)

Abstract

Until now, only few attempts have been made to assess biofilm models simulating microenvironments in a biofilm. As a first step, we compare the microenvironment observed in a membrane aerated biofilm (MAB) to that derived from a two-dimensional computational model with individual ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) embedded in a continuum EPS matrix. Gradients of oxygen were determined by means of microelectrodes. The change in nitrifying bacterial populations with the biofilm depth was quantified using fluorescence in situ hybridization (FISH) in combination with a confocal laser scanning microscopy (CLSM). Microelectrode measurements revealed that oxic and anoxic or anaerobic regions exist within the MAB. The oxygen profile predicted by the model showed good agreement with that obtained by microelectrode measurements. The oxic part of the biofilm was dominated by NSO190 probe-hybridized AOB, which formed relatively large clusters of cells directly on the membrane surface, and by the NOB belonging to genus Nitrobacter sp. On the other hand, NOB belonging to genus Nitrospira sp. were abundant at the oxic-anoxic interface. The model prediction regarding AOB and Nitrobacter sp. distribution was consistent with the experimental counterpart. Measurements of AOB cluster size distribution showed that colonies are slightly larger adjacent to the membrane than at the inner part of the biofilm. The sizes predicted by the current model are larger than those obtained in the experiment, leading to the arguments that some factors not contained in the model would affect the cluster size.

Original languageEnglish
Title of host publicationBiofilm Systems VI
EditorsM.C.M. Loosdrecht, C. Picioreau
Pages283-290
Number of pages8
Edition8-9
DOIs
Publication statusPublished - 2007 Jun 5

Publication series

NameWater Science and Technology
Number8-9
Volume55
ISSN (Print)0273-1223

Keywords

  • Biofilm model
  • Fluorescence in sity hybridization (FISH)
  • Membrane-aerated biofilm reactor (MABR)
  • Two-dimensional

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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    Matsumoto, S., Terada, A., Aoi, Y., Tsuneda, S., Alpkvist, E., Picioreanu, C., & van Loosdrecht, M. C. M. (2007). Experimental and simulation analysis of community structure of nitrifying bacteria in a membrane-aerated biofilm. In M. C. M. Loosdrecht, & C. Picioreau (Eds.), Biofilm Systems VI (8-9 ed., pp. 283-290). (Water Science and Technology; Vol. 55, No. 8-9). https://doi.org/10.2166/wst.2007.269