Experimental Evidence for the Existence of a Stable Half-Barrel Subdomain in the (β/α)8-Barrel Fold

Satoshi Akanuma, Akihiko Yamagishi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The (β/α)8-barrel is one of the most common folds functioning as enzymes. The emergence of two (β/α)8-barrel enzymes involved in histidine biosynthesis, each of which has a twofold symmetric structure, has been proposed to be a consequence of tandem duplication and fusion of a (β/α)4-half-barrel. However, little evidence has been found for the existence of an ancestral half-barrel in the evolution of other (β/α)8-barrel proteins. In order to detect remnants of an ancestral half-barrel in the (β/α)8-barrel structure of Escherichia coli N-(5′-phosphoribosyl)anthranilate isomerase, we engineered three potential half-barrel units, (β/α)1-4, (β/α)3-6, and (β/α)5-8. Among these three arrangements, only (β/α)3-6 is stable; it exists in equilibrium between monomeric and dimeric forms. Thus, the central segment of N-(5′-phosphoribosyl)anthranilate isomerase from E. coli can serve as a half-barrel precursor. A tandem duplication of (β/α)3-6 yielded predominantly monomeric structures that were quite stable. This result exemplified that the structural characteristics of noncovalently assembled half-barrels could be improved by duplication and fusion. Moreover, our results may provide information regarding the local structural units that encompass interactions important for the early folding events of this ubiquitous protein conformation.

Original languageEnglish
Pages (from-to)458-466
Number of pages9
JournalJournal of Molecular Biology
Volume382
Issue number2
DOIs
Publication statusPublished - 2008 Oct 3
Externally publishedYes

Fingerprint

Isomerases
Escherichia coli
Protein Conformation
Enzymes
Histidine
Proteins
anthranilic acid

Keywords

  • (β/α)-barrel
  • gene duplication
  • half-barrel
  • protein stability
  • tryptophan biosynthetic enzyme

ASJC Scopus subject areas

  • Virology

Cite this

Experimental Evidence for the Existence of a Stable Half-Barrel Subdomain in the (β/α)8-Barrel Fold. / Akanuma, Satoshi; Yamagishi, Akihiko.

In: Journal of Molecular Biology, Vol. 382, No. 2, 03.10.2008, p. 458-466.

Research output: Contribution to journalArticle

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