Exposure of leaves of Cucumis sativus L. To low temperatures in the light causes uncoupling of thylakoids II. Non-destructive measurements with intact leaves

Ichiro Terashima, Kintake Sonoike, Tamotsu Kawazu, Sakae Katoh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To examine the effects of chilling of leaves of cucumber (Cucumis sativus L.) in moderate light on the coupling state of thylakoids in situ, changes in fluorescence, changes in light scattering and flash-induced changes in absorbance at 518 nm were examined in intact leaves. After chilling of leaves at 5°C in the light for 5 h, the non-photochemical quenching of fluorescence, a measure of energisation of thylakoids, was largely suppressed. The treatment also caused a suppression of light-induced changes in the light scattering by leaves, which depends on the formation of a pH gradient across thylakoid membranes. When thylakoids were prepared by very gentle methods from the leaves chilled in the light, through a step of preparation of intact chloro-plasts, the transport of electrons from H2O to ferricyanide was uncoupled, being insensitive to an uncoupler, methylamine.These data provide consistent evidence that the thylakoids are uncoupled in situ by the chilling of leaves in the light and, as a consequence of the uncoupling, the energisation of the membranes is suppressed. However, the decay of the flash-induced change in absorbance at 518 nm in leaves was not markedly accelerated by the treatment. The thylakoids isolated from leaves chilled in the light, which were in the uncoupled state, also did not show a rapid decay, unless an efficient uncoupler such as gramicidin was added. These results suggest that even a considerable uncoupling of thylakoids, brought about by chilling of leaves in the light, is not sufficient to cause a marked acceleration of the decay of the flash-induced change in absorbance at 518 nm. Therefore, analysis at 518 nm is not always a sensitive method for assessing the coupling state of thylakoids.

Original languageEnglish
Pages (from-to)1275-1283
Number of pages9
JournalPlant and Cell Physiology
Volume32
Issue number8
Publication statusPublished - 1991 Dec
Externally publishedYes

Fingerprint

Cucumis Sativus
Cucumis sativus
Thylakoids
thylakoids
Leaves
Chilling
Light
Temperature
leaves
temperature
Flash
absorbance
Light scattering
deterioration
Light Scattering
light scattering
Decay
Fluorescence
Membranes
fluorescence

Keywords

  • ATPase
  • Chilling stress
  • Cucumis sativus L. (Cucurbitaceae)
  • Photosynthesis
  • Thylakoids
  • Uncoupling

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Exposure of leaves of Cucumis sativus L. To low temperatures in the light causes uncoupling of thylakoids II. Non-destructive measurements with intact leaves. / Terashima, Ichiro; Sonoike, Kintake; Kawazu, Tamotsu; Katoh, Sakae.

In: Plant and Cell Physiology, Vol. 32, No. 8, 12.1991, p. 1275-1283.

Research output: Contribution to journalArticle

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abstract = "To examine the effects of chilling of leaves of cucumber (Cucumis sativus L.) in moderate light on the coupling state of thylakoids in situ, changes in fluorescence, changes in light scattering and flash-induced changes in absorbance at 518 nm were examined in intact leaves. After chilling of leaves at 5°C in the light for 5 h, the non-photochemical quenching of fluorescence, a measure of energisation of thylakoids, was largely suppressed. The treatment also caused a suppression of light-induced changes in the light scattering by leaves, which depends on the formation of a pH gradient across thylakoid membranes. When thylakoids were prepared by very gentle methods from the leaves chilled in the light, through a step of preparation of intact chloro-plasts, the transport of electrons from H2O to ferricyanide was uncoupled, being insensitive to an uncoupler, methylamine.These data provide consistent evidence that the thylakoids are uncoupled in situ by the chilling of leaves in the light and, as a consequence of the uncoupling, the energisation of the membranes is suppressed. However, the decay of the flash-induced change in absorbance at 518 nm in leaves was not markedly accelerated by the treatment. The thylakoids isolated from leaves chilled in the light, which were in the uncoupled state, also did not show a rapid decay, unless an efficient uncoupler such as gramicidin was added. These results suggest that even a considerable uncoupling of thylakoids, brought about by chilling of leaves in the light, is not sufficient to cause a marked acceleration of the decay of the flash-induced change in absorbance at 518 nm. Therefore, analysis at 518 nm is not always a sensitive method for assessing the coupling state of thylakoids.",
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