Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

Masahiro Ishiura, Shinsuke Kutsuna, Setsuyuki Aoki, Hideo Iwasaki, Carol R. Andersson, Akio Tanabe, Susan S. Golden, Carl H. Johnson, Takao Kondo

Research output: Contribution to journalArticle

515 Citations (Scopus)

Abstract

Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.

Original languageEnglish
Pages (from-to)1519-1523
Number of pages5
JournalScience
Volume281
Issue number5382
DOIs
Publication statusPublished - 1998 Sep 4
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria'. Together they form a unique fingerprint.

  • Cite this

    Ishiura, M., Kutsuna, S., Aoki, S., Iwasaki, H., Andersson, C. R., Tanabe, A., Golden, S. S., Johnson, C. H., & Kondo, T. (1998). Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science, 281(5382), 1519-1523. https://doi.org/10.1126/science.281.5382.1519