Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

Masahiro Ishiura; Shinsuke Kutsuna; Setsuyuki Aoki; Hideo Iwasaki; Carol R. Andersson; Akio Tanabe; Susan S. Golden; Carl H. Johnson; Takao Kondo

doi:10.1126/science.281.5382.1519

Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

Masahiro Ishiura^*, Shinsuke Kutsuna, Setsuyuki Aoki, Hideo Iwasaki, Carol R. Andersson, Akio Tanabe, Susan S. Golden, Carl H. Johnson, Takao Kondo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

578 Citations (Scopus)

Abstract

Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.

Original language	English
Pages (from-to)	1519-1523
Number of pages	5
Journal	Science
Volume	281
Issue number	5382
DOIs	https://doi.org/10.1126/science.281.5382.1519
Publication status	Published - 1998 Sept 4
Externally published	Yes

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title = "Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria",

abstract = "Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.",

author = "Masahiro Ishiura and Shinsuke Kutsuna and Setsuyuki Aoki and Hideo Iwasaki and Andersson, {Carol R.} and Akio Tanabe and Golden, {Susan S.} and Johnson, {Carl H.} and Takao Kondo",

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doi = "10.1126/science.281.5382.1519",

language = "English",

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TY - JOUR

T1 - Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

AU - Ishiura, Masahiro

AU - Kutsuna, Shinsuke

AU - Aoki, Setsuyuki

AU - Iwasaki, Hideo

AU - Andersson, Carol R.

AU - Tanabe, Akio

AU - Golden, Susan S.

AU - Johnson, Carl H.

AU - Kondo, Takao

PY - 1998/9/4

Y1 - 1998/9/4

N2 - Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.

AB - Cyanobacteria are the simplest organisms known to have a circadian clock. A circadian clock gene cluster kaiABC was cloned from the cyanobacterium Synechococcus. Nineteen clock mutations were mapped to the three kai genes. Promoter activities upstream of the kaiA and kaiB genes showed circadian rhythms of expression, and both kaiA and kaiBC messenger RNAs displayed circadian cycling. Inactivation of any single kai gene abolished these rhythms and reduced kaiBC-promoter activity. Continuous kaiC overexpression repressed the kaiBC promoter, whereas kaiA overexpression enhanced it. Temporal kaiC overexpression reset the phase of the rhythms. Thus, a negative feedback control of kaiC expression by KaiC generates a circadian oscillation in cyanobacteria, and KaiA sustains the oscillation by enhancing kaiC expression.

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Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria

Abstract

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