Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea

H. H. Seong, K. Shiota, K. Noda, A. Ogura, T. Asano, M. Takahashi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The rat ovary contains two isozymes of 20α-hydroxysteroid dehydrogenase (HSD-1 and HSD-2). In this study, the expression of activity of each isozyme was investigated in ovaries that contained a single generation of corpora lutea during pseudopregnancy. This condition was induced by cervical stimulation in rats that had been rendered anovulatory by housing them in a continuously lit environment. The total activity of cytosolic 20α-HSD was lower in the ovaries of these pseudopregnant rats than in ovaries containing multiple generations of corpora lutea. In normal pseudopregnancy, HSD-1 activity was low on days 5 and 9 and increased markedly on day 15, whereas HSD-2 was lower than HSD-1 and did not vary throughout pseudopregnancy. However, on days 5 and 9 of continuous-light pseudopregnancy, low activity of HSD-1 only was detected; by day 15, HSD-1 activity had increased sixfold and HSD-2 activity could be detected. Immunohistochemical methods using a specific antibody recognizing both HSD-1 and HSD-2 revealed that the number of 20α-HSD-positive luteal cells increased by day 15. Thus, the increase in total enzyme activity and appearance of HSD-2 activity observed at late pseudopregnancy was accompanied by an increase in the number of 20α-HSD-positive luteal cells.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalJournal of Reproduction and Fertility
Volume96
Issue number2
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

20-Hydroxysteroid Dehydrogenases
Pseudopregnancy
Corpus Luteum
Isoenzymes
Ovary
Luteal Cells
Light
Antibodies
Enzymes

Keywords

  • 20α-hydroxysteroid dehydrogenase
  • Corpus luteum
  • Pseudopregnancy
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Embryology
  • Obstetrics and Gynaecology

Cite this

Seong, H. H., Shiota, K., Noda, K., Ogura, A., Asano, T., & Takahashi, M. (1992). Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea. Journal of Reproduction and Fertility, 96(2), 573-580.

Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea. / Seong, H. H.; Shiota, K.; Noda, K.; Ogura, A.; Asano, T.; Takahashi, M.

In: Journal of Reproduction and Fertility, Vol. 96, No. 2, 1992, p. 573-580.

Research output: Contribution to journalArticle

Seong, HH, Shiota, K, Noda, K, Ogura, A, Asano, T & Takahashi, M 1992, 'Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea', Journal of Reproduction and Fertility, vol. 96, no. 2, pp. 573-580.
Seong, H. H. ; Shiota, K. ; Noda, K. ; Ogura, A. ; Asano, T. ; Takahashi, M. / Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea. In: Journal of Reproduction and Fertility. 1992 ; Vol. 96, No. 2. pp. 573-580.
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