Expression of amoA mRNA in wastewater treatment processes examined by competitive RT-PCR

Yoshiteru Aoi, Yuko Shiramasa, Youske Masaki, Satoshi Tsuneda, Akira Hirata, Atsuhi Kitayama, Teruyuki Nagamune

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    10 Citations (Scopus)

    Abstract

    The expression of ammonia monooxygenase encoding mRNA (amoA mRNA) in a wastewater treatment process was analyzed in an attempt to propose an effective target for the monitoring of nitrifying bacteria in engineered systems or natural environments. The quick response (1-2 h) of amoA mRNA transcription to the recovery of ammonia oxidation activity induced by the sudden exposure to ammonia was observed in a short-time batch-mode incubation whereas the amount of amoA DNA did not markedly change during the incubation under any conditions. In the continuous feeding-operation, amoA mRNA level dynamically changed in response to the change in the surrounding environmental conditions and increase in ammonia oxidation rate. Although, amoA mRNA level did not quickly respond to the decrease in ammonia oxidation activity, it decreases over long time scales. These results suggest that the profiles of amoA mRNA expression can be used as an indicator of the ammonia oxidation activity.

    Original languageEnglish
    Pages (from-to)111-120
    Number of pages10
    JournalJournal of Biotechnology
    Volume111
    Issue number2
    DOIs
    Publication statusPublished - 2004 Jul 15

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    Keywords

    • Ammonia monooxigenase (AMO)
    • Ammonia-oxidizing bacteria
    • amoA mRNA
    • Competitive RT-PCR
    • Nitrification

    ASJC Scopus subject areas

    • Biotechnology

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