Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

Yoichi Ito, Atsushi Hirota, Mariko Nakamura, Masahiro Matsunaga, Kazuyoshi Tsutsui, Sakaé Kikuyama

Research output: Contribution to journalArticle

Abstract

As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.

Original languageEnglish
Pages (from-to)348-350
Number of pages3
JournalAnnals of the New York Academy of Sciences
Volume1040
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Salamandridae
Aromatase
Aromatization
Messenger RNA
Testosterone
Complementary DNA
Cloning
Gene Library
Cytochrome P-450 Enzyme System
In Situ Hybridization
Organism Cloning
Estradiol
High Pressure Liquid Chromatography
Polymerase Chain Reaction
In Situ
High-performance Liquid Chromatography
Thin-layer Chromatography
Hybridization

Keywords

  • Abdominal gland
  • Aromatase
  • Newt

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster. / Ito, Yoichi; Hirota, Atsushi; Nakamura, Mariko; Matsunaga, Masahiro; Tsutsui, Kazuyoshi; Kikuyama, Sakaé.

In: Annals of the New York Academy of Sciences, Vol. 1040, 2005, p. 348-350.

Research output: Contribution to journalArticle

Ito, Yoichi ; Hirota, Atsushi ; Nakamura, Mariko ; Matsunaga, Masahiro ; Tsutsui, Kazuyoshi ; Kikuyama, Sakaé. / Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster. In: Annals of the New York Academy of Sciences. 2005 ; Vol. 1040. pp. 348-350.
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