Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

Yoichi Ito; Atsushi Hirota; Mariko Nakamura; Masahiro Matsunaga; Kazuyoshi Tsutsui; Sakaé Kikuyama

doi:10.1196/annals.1327.058

Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

Yoichi Ito, Atsushi Hirota, Mariko Nakamura, Masahiro Matsunaga, Kazuyoshi Tsutsui, Sakaé Kikuyama^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.

Original language	English
Pages (from-to)	348-350
Number of pages	3
Journal	Annals of the New York Academy of Sciences
Volume	1040
DOIs	https://doi.org/10.1196/annals.1327.058
Publication status	Published - 2005
Externally published	Yes

Keywords

Abdominal gland
Aromatase
Newt

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
History and Philosophy of Science

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10.1196/annals.1327.058

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abstract = "As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.",

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doi = "10.1196/annals.1327.058",

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T1 - Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

AU - Ito, Yoichi

AU - Hirota, Atsushi

AU - Nakamura, Mariko

AU - Matsunaga, Masahiro

AU - Tsutsui, Kazuyoshi

AU - Kikuyama, Sakaé

PY - 2005

Y1 - 2005

N2 - As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.

AB - As a step to see whether the local aromatization mechanism exists in the newt, Cynops pyrrhogaster, cDNA cloning and characterization of newt cytochrome P450 aromatase (P450arom) were conducted. Newt P450arom cDNA was obtained from a C. pyrrhogaster ovarian cDNA library. By RT-PCR and in situ hybridization, an intense P450arom mRNA expression was detected in the abdominal gland. Employing biochemical techniques combined with HPLC and TLC analyses, we also demonstrated the conversion of testosterone to estradiol in the abdominal grand. The significance of aromatization of testosterone in this gland is discussed.

KW - Abdominal gland

KW - Aromatase

KW - Newt

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Expression of aromatase mRNA in the abdominal gland of the newt, Cynops pyrrhogaster

Abstract

Keywords

ASJC Scopus subject areas

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