Expression of Basic Fibroblast Growth Factor-Like Substance in Immature Rat Testis: Immunocytochemical Localization and Detection of mRNA

Takayuki Kamei, Katsushi Suzuki, Kunio Shiota, Tomoya Ogawa, Michio Takahashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have previously described immunoreactive basic fibroblast growth factor (bFGF)-like substance in adult rat testis and have shown that this bFGF-like substance presents in the nucleus of germ cells. In the present study, the distribution of bFGF was examined immunohistochemically in 3 or 12-day-old rat testes. In 3-day-old, immunoreactivity for bFGF was detected in the cytoplasm of gonocytes (prespermatogonia) and fetal Leydig cells. In contrast, in 12-day-old, immunoreactivity for bFGF was detected exclusively in the nucleus of germ cells. Thus, bFGF seems to translocate from the cytoplasm to the nucleus during formation of the first wave of spermatogenesis. Using reverse transcription-polymerase chain reaction (RT-PCR), bFGF mRNA was detected in the 3-day-old rat whole testis. Moreover, in situ hybridization analysis revealed gonocytes and fetal Leydig cells contain mRNA for bFGF. These results indicate that bFGF is synthesized in gonocytes and fetal Leydig cells before the formation of the first wave of spermatogenesis.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Reproduction and Development
Volume39
Issue number1
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

fibroblast growth factor 2
testes
immatures
rats
Leydig cells
spermatogenesis
germ cells
cytoplasm
in situ hybridization
reverse transcriptase polymerase chain reaction

Keywords

  • Basic fibroblast growth factor
  • Germ cells
  • Leydig cells
  • Nuclear translocation

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Expression of Basic Fibroblast Growth Factor-Like Substance in Immature Rat Testis : Immunocytochemical Localization and Detection of mRNA. / Kamei, Takayuki; Suzuki, Katsushi; Shiota, Kunio; Ogawa, Tomoya; Takahashi, Michio.

In: Journal of Reproduction and Development, Vol. 39, No. 1, 1993, p. 33-39.

Research output: Contribution to journalArticle

Kamei, Takayuki ; Suzuki, Katsushi ; Shiota, Kunio ; Ogawa, Tomoya ; Takahashi, Michio. / Expression of Basic Fibroblast Growth Factor-Like Substance in Immature Rat Testis : Immunocytochemical Localization and Detection of mRNA. In: Journal of Reproduction and Development. 1993 ; Vol. 39, No. 1. pp. 33-39.
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