Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus

Takehiko Mima, Kazuyoshi Gotoh, Yumiko Yamamoto, Keiko Maeda, Taku Shirakawa, Shunsuke Matsui, Yumi Murata, Takaki Koide, Hiroshi Tokumitsu, Osamu Matsushita

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Vibrio alginolyticus is an opportunistic pathogen in both humans and marine animals. Collagenase encoded by colA is considered to be one of the virulence factors. Expression of colA is regulated by multiple environmental factors, e.g., temperature, growth phase, and substrate. To elucidate the mechanism of regulation of colA expression, transposon mutagenesis was performed. VarS, a sensor histidine kinase of the two-component regulatory system, was demonstrated to regulate the expression of colA. VarA, a cognate response regulator of VarS, was also identified and shown to be involved in the regulation of colA expression. In vitro phosphorylation assays showed that phosphorylated VarS acted as a phosphoryl group donor to VarA. A site-directed mutagenesis study showed that the His300, Asp718 and His874 residues in VarS were essential for the phosphorylation of VarS, and the Asp54 residue in VarA was likely to receive the phosphoryl group from VarS. The results demonstrate that the VarS/VarA two-component regulatory system regulates the expression of collagenase in V. alginolyticus.

    Original languageEnglish
    Pages (from-to)1-13
    Number of pages13
    JournalJournal of Membrane Biology
    DOIs
    Publication statusAccepted/In press - 2017 Oct 9

    Fingerprint

    Vibrio alginolyticus
    Colicins
    Collagenases
    Phosphorylation
    Virulence Factors
    Site-Directed Mutagenesis
    Mutagenesis
    Temperature
    Growth

    Keywords

    • Collagenase
    • Two-component regulatory system
    • Vibrio alginolyticus

    ASJC Scopus subject areas

    • Biophysics
    • Physiology
    • Cell Biology

    Cite this

    Mima, T., Gotoh, K., Yamamoto, Y., Maeda, K., Shirakawa, T., Matsui, S., ... Matsushita, O. (Accepted/In press). Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. Journal of Membrane Biology, 1-13. https://doi.org/10.1007/s00232-017-9991-9

    Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. / Mima, Takehiko; Gotoh, Kazuyoshi; Yamamoto, Yumiko; Maeda, Keiko; Shirakawa, Taku; Matsui, Shunsuke; Murata, Yumi; Koide, Takaki; Tokumitsu, Hiroshi; Matsushita, Osamu.

    In: Journal of Membrane Biology, 09.10.2017, p. 1-13.

    Research output: Contribution to journalArticle

    Mima, T, Gotoh, K, Yamamoto, Y, Maeda, K, Shirakawa, T, Matsui, S, Murata, Y, Koide, T, Tokumitsu, H & Matsushita, O 2017, 'Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus', Journal of Membrane Biology, pp. 1-13. https://doi.org/10.1007/s00232-017-9991-9
    Mima, Takehiko ; Gotoh, Kazuyoshi ; Yamamoto, Yumiko ; Maeda, Keiko ; Shirakawa, Taku ; Matsui, Shunsuke ; Murata, Yumi ; Koide, Takaki ; Tokumitsu, Hiroshi ; Matsushita, Osamu. / Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. In: Journal of Membrane Biology. 2017 ; pp. 1-13.
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