Expression of DMRT genes in the gonads of rana rugosa during sex determination

Yusaku Matsushita, Yuki Oshima, Masahisa Nakamura

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Sex is determined by various transcription factors. Dmrt1, a gene that contains the DM domain (Doublesex/Mab-3 DNA-binding motif), is known to be one of the critical factors required for testicular development in vertebrates. Recently, other DM domain-containing genes (DMRT genes) were isolated in mice and fish. In mice, three of the seven known DMRT genes are expressed in the embryonic gonad. In amphibians, however, only Dmrt1 and Dmrt4 have been cloned. To clarify the role(s) of DMRT genes in sex determination in vertebrates, the molecular cloning of amphibian DMRT genes would be very helpful. In this study, we isolated Dmrt2, -3, and -5 from the frog Rana rugosa and analyzed by RT-PCR their expression during sex determination. The analysis revealed that all three genes are expressed in the developing gonad/mesonephros complex during sex determination with no dimorphism. The results suggest that Dmrt2, -3, and -5 may be involved in sexual development in R. rugosa and that they may function in both testicular and ovarian differentiation.

    Original languageEnglish
    Pages (from-to)95-99
    Number of pages5
    JournalZoological Science
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - 2007 Jan

    Fingerprint

    gonads
    gene expression
    gender
    genes
    amphibians
    vertebrates
    testicular development
    DNA-binding domains
    sexual development
    mice
    Glandirana rugosa
    dimorphism
    frogs
    molecular cloning
    transcription factors
    reverse transcriptase polymerase chain reaction
    fish

    Keywords

    • DMRT genes
    • Frog
    • Gonad
    • Rana rugosa
    • RT-PCR
    • Sex determination

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Expression of DMRT genes in the gonads of rana rugosa during sex determination. / Matsushita, Yusaku; Oshima, Yuki; Nakamura, Masahisa.

    In: Zoological Science, Vol. 24, No. 1, 01.2007, p. 95-99.

    Research output: Contribution to journalArticle

    Matsushita, Yusaku ; Oshima, Yuki ; Nakamura, Masahisa. / Expression of DMRT genes in the gonads of rana rugosa during sex determination. In: Zoological Science. 2007 ; Vol. 24, No. 1. pp. 95-99.
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