Expression of Dmrt1 protein in developing and in sex-reversed gonads of amphibians

S. Aoyama, K. Shibata, S. Tokunaga, M. Takase, K. Matsui, M. Nakamura

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    Many genes are known to be involved in gonadal differentiation in vertebrates. Dmrt1, a gene that encodes a transcription factor with a DM-domain, is considered to be one of the essential genes controlling testicular differentiation in mammals, birds, reptiles, amphibians and fish. However, it still remains unknown which testicular cells of animals other than mice and chicks express Dmrt1 protein. For an explanation of its role(s) in testicular differentiation in vertebrates, the expression of the Dmrt1 protein needs to be studied. For this purpose, we conducted an immunohistochemical study of this protein in an amphibian by using an antibody specific for Dmrt1. No positive signal was found in the indifferent gonad of tadpoles of Rana rugosa at early stages. However, in the testis of tadpoles at later stages (XV-XXV) and in frogs one month after metamorphosis, this protein was expressed in interstitial cells and Sertoli cells. In the testis of adult frogs, germ cells also expressed Dmrt1 protein. RT-PCR analysis revealed that the gene for this protein was not transcribed at any time during ovarian development, but was expressed in the female to male sex-reversed gonad. This was true when immunohistological studies were performed. In addition, Southern blot analysis showed Dmrt1 to be an autosomal gene. Taken together, our findings indicate that Dmrt1 protein is expressed by interstitial cells, Seroli cells and germ cells in the testis of R. rugosa. Dmrt1 may thus be very involved in the testicular differentiation of amphibians.

    Original languageEnglish
    Pages (from-to)295-301
    Number of pages7
    JournalCytogenetic and Genome Research
    Volume101
    Issue number3-4
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Gonads
    Amphibians
    Testis
    Proteins
    Germ Cells
    Anura
    Amphibian Proteins
    Larva
    Vertebrates
    Genes
    Ranidae
    Reptiles
    Sertoli Cells
    Essential Genes
    Southern Blotting
    Birds
    Mammals
    Fishes
    Transcription Factors
    Polymerase Chain Reaction

    ASJC Scopus subject areas

    • Genetics
    • Cell Biology

    Cite this

    Aoyama, S., Shibata, K., Tokunaga, S., Takase, M., Matsui, K., & Nakamura, M. (2003). Expression of Dmrt1 protein in developing and in sex-reversed gonads of amphibians. Cytogenetic and Genome Research, 101(3-4), 295-301. https://doi.org/10.1159/000074352

    Expression of Dmrt1 protein in developing and in sex-reversed gonads of amphibians. / Aoyama, S.; Shibata, K.; Tokunaga, S.; Takase, M.; Matsui, K.; Nakamura, M.

    In: Cytogenetic and Genome Research, Vol. 101, No. 3-4, 2003, p. 295-301.

    Research output: Contribution to journalArticle

    Aoyama, S, Shibata, K, Tokunaga, S, Takase, M, Matsui, K & Nakamura, M 2003, 'Expression of Dmrt1 protein in developing and in sex-reversed gonads of amphibians', Cytogenetic and Genome Research, vol. 101, no. 3-4, pp. 295-301. https://doi.org/10.1159/000074352
    Aoyama, S. ; Shibata, K. ; Tokunaga, S. ; Takase, M. ; Matsui, K. ; Nakamura, M. / Expression of Dmrt1 protein in developing and in sex-reversed gonads of amphibians. In: Cytogenetic and Genome Research. 2003 ; Vol. 101, No. 3-4. pp. 295-301.
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