Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Yoshikazu Higami, Tomoshi Tsuchiya, Kazuo To, Takuya Chiba, Haruyoshi Yamaza, Daisuke Shiokawa, Sei Ichi Tanuma, Isao Shimokawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

Original languageEnglish
Pages (from-to)403-407
Number of pages5
JournalCell and Tissue Research
Volume316
Issue number3
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Fingerprint

Deoxyribonucleases
DNA Fragmentation
Hepatocytes
Rodentia
DNA
Endonucleases
Cycloheximide
Labeling
Lipopolysaccharides
Necrosis
Apoptosis
Carbon Tetrachloride
Liver
Paraffin
Immunohistochemistry
deoxyribonuclease gamma
Antibodies

Keywords

  • Apoptosis
  • Cycloheximide
  • DNase gamma
  • Fas
  • Hepatocytes
  • Mouse (BALB/c)
  • Rat (F344)

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. / Higami, Yoshikazu; Tsuchiya, Tomoshi; To, Kazuo; Chiba, Takuya; Yamaza, Haruyoshi; Shiokawa, Daisuke; Tanuma, Sei Ichi; Shimokawa, Isao.

In: Cell and Tissue Research, Vol. 316, No. 3, 06.2004, p. 403-407.

Research output: Contribution to journalArticle

Higami, Y, Tsuchiya, T, To, K, Chiba, T, Yamaza, H, Shiokawa, D, Tanuma, SI & Shimokawa, I 2004, 'Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes', Cell and Tissue Research, vol. 316, no. 3, pp. 403-407. https://doi.org/10.1007/s00441-004-0890-x
Higami, Yoshikazu ; Tsuchiya, Tomoshi ; To, Kazuo ; Chiba, Takuya ; Yamaza, Haruyoshi ; Shiokawa, Daisuke ; Tanuma, Sei Ichi ; Shimokawa, Isao. / Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. In: Cell and Tissue Research. 2004 ; Vol. 316, No. 3. pp. 403-407.
@article{c950f40649fc4145b55996f91913f729,
title = "Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes",
abstract = "Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.",
keywords = "Apoptosis, Cycloheximide, DNase gamma, Fas, Hepatocytes, Mouse (BALB/c), Rat (F344)",
author = "Yoshikazu Higami and Tomoshi Tsuchiya and Kazuo To and Takuya Chiba and Haruyoshi Yamaza and Daisuke Shiokawa and Tanuma, {Sei Ichi} and Isao Shimokawa",
year = "2004",
month = "6",
doi = "10.1007/s00441-004-0890-x",
language = "English",
volume = "316",
pages = "403--407",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

AU - Higami, Yoshikazu

AU - Tsuchiya, Tomoshi

AU - To, Kazuo

AU - Chiba, Takuya

AU - Yamaza, Haruyoshi

AU - Shiokawa, Daisuke

AU - Tanuma, Sei Ichi

AU - Shimokawa, Isao

PY - 2004/6

Y1 - 2004/6

N2 - Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

AB - Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

KW - Apoptosis

KW - Cycloheximide

KW - DNase gamma

KW - Fas

KW - Hepatocytes

KW - Mouse (BALB/c)

KW - Rat (F344)

UR - http://www.scopus.com/inward/record.url?scp=3042545921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042545921&partnerID=8YFLogxK

U2 - 10.1007/s00441-004-0890-x

DO - 10.1007/s00441-004-0890-x

M3 - Article

VL - 316

SP - 403

EP - 407

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 3

ER -