Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Yoshikazu Higami; Tomoshi Tsuchiya; Kazuo To; Takuya Chiba; Haruyoshi Yamaza; Daisuke Shiokawa; Sei Ichi Tanuma; Isao Shimokawa

doi:10.1007/s00441-004-0890-x

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Yoshikazu Higami^*, Tomoshi Tsuchiya, Kazuo To, Takuya Chiba, Haruyoshi Yamaza, Daisuke Shiokawa, Sei Ichi Tanuma, Isao Shimokawa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl₄). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl₄-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

Original language	English
Pages (from-to)	403-407
Number of pages	5
Journal	Cell and Tissue Research
Volume	316
Issue number	3
DOIs	https://doi.org/10.1007/s00441-004-0890-x
Publication status	Published - 2004 Jun
Externally published	Yes

Keywords

Apoptosis
Cycloheximide
DNase gamma
Fas
Hepatocytes
Mouse (BALB/c)
Rat (F344)

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology
Cell Biology

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@article{c950f40649fc4145b55996f91913f729,

title = "Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes",

abstract = "Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.",

keywords = "Apoptosis, Cycloheximide, DNase gamma, Fas, Hepatocytes, Mouse (BALB/c), Rat (F344)",

author = "Yoshikazu Higami and Tomoshi Tsuchiya and Kazuo To and Takuya Chiba and Haruyoshi Yamaza and Daisuke Shiokawa and Tanuma, {Sei Ichi} and Isao Shimokawa",

year = "2004",

month = jun,

doi = "10.1007/s00441-004-0890-x",

language = "English",

volume = "316",

pages = "403--407",

journal = "Cell and Tissue Research",

issn = "0302-766X",

publisher = "Springer Verlag",

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TY - JOUR

T1 - Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

AU - Higami, Yoshikazu

AU - Tsuchiya, Tomoshi

AU - To, Kazuo

AU - Chiba, Takuya

AU - Yamaza, Haruyoshi

AU - Shiokawa, Daisuke

AU - Tanuma, Sei Ichi

AU - Shimokawa, Isao

PY - 2004/6

Y1 - 2004/6

N2 - Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

AB - Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

KW - Apoptosis

KW - Cycloheximide

KW - DNase gamma

KW - Fas

KW - Hepatocytes

KW - Mouse (BALB/c)

KW - Rat (F344)

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DO - 10.1007/s00441-004-0890-x

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AN - SCOPUS:3042545921

SN - 0302-766X

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EP - 407

JO - Cell and Tissue Research

JF - Cell and Tissue Research

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ER -

Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Abstract

Keywords

ASJC Scopus subject areas

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