Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Yoshikazu Higami, Tomoshi Tsuchiya, Kazuo To, Takuya Chiba, Haruyoshi Yamaza, Daisuke Shiokawa, Sei Ichi Tanuma, Isao Shimokawa

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6 Citations (Scopus)

Abstract

Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase y was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase y immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase y immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase y plays an important role in Fasindependent apoptotic DNA fragmentation in hepatocytes.

Original languageEnglish
Pages (from-to)403-407
Number of pages5
JournalCell and Tissue Research
Volume316
Issue number3
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Keywords

  • Apoptosis
  • Cycloheximide
  • DNase gamma
  • Fas
  • Hepatocytes
  • Mouse (BALB/c)
  • Rat (F344)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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  • Cite this

    Higami, Y., Tsuchiya, T., To, K., Chiba, T., Yamaza, H., Shiokawa, D., Tanuma, S. I., & Shimokawa, I. (2004). Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. Cell and Tissue Research, 316(3), 403-407. https://doi.org/10.1007/s00441-004-0890-x