Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form

Youichi Aizawa, Nami Nogawa, Nobuyoshi Kosaka, Yasutaka Maeda, Takafumi Watanabe, Hiroshi Miyazaki, Takashi Kato

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The regulation of hematopoiesis in non-mammalian vertebrates is poorly understood. This is partly because the structures and effects of most hematopoietic regulators have not been identified. As a first step towards studies on the key mechanism of hematopoietic regulation among phyla as well as the diversity of organisms, we have focused on amphibian hematopoiesis. A cDNA sharing the highest degree of homology with mammalian erythropoietin (EPO) receptors, tentatively named xlEPOR, was cloned from a cDNA library of Xenopus laevis immature erythrocytes. The comparative identities of the deduced entire amino acid sequence to mammalian EPO receptors were quite low, although functional domains indispensable for erythropoietic activities were found in the molecule. Northern analysis revealed that xlEPOR were expressed in peripheral blood cells. In the peripheral blood of phenylhydrazine-treated adult Xenopus, immature erythrocytes expressing xlEPOR were identified by in situ hybridization and immunostaining with polyclonal antibodies to xlEPOR. To confirm the biological functions of this molecule, the extracellular domain of xlEPOR (i.e., soluble xlEPOR) was administered to adult Xenopus by consecutive intracardiac injection. The peripheral erythrocyte counts were decreased gradually; meanwhile, immature erythrocytes appeared in the circulation, demonstrating that xlEPOR plays a significant physiological role in erythropoiesis in Xenopus laevis.

    Original languageEnglish
    Pages (from-to)167-175
    Number of pages9
    JournalJournal of Biochemistry
    Volume138
    Issue number2
    DOIs
    Publication statusPublished - 2005 Aug

    Fingerprint

    Erythropoietin Receptors
    Xenopus laevis
    Blood
    Erythrocytes
    Hematopoiesis
    Xenopus
    Molecules
    Gene Library
    Erythrocyte Count
    Complementary DNA
    Erythropoiesis
    Cells
    Amphibians
    Amino Acids
    In Situ Hybridization
    Vertebrates
    Antibodies
    Amino Acid Sequence
    Blood Cells
    Injections

    Keywords

    • Erythrocyte
    • Erythropoieisis
    • Erythropoietin receptor
    • Soluble receptor
    • Xenopus

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form. / Aizawa, Youichi; Nogawa, Nami; Kosaka, Nobuyoshi; Maeda, Yasutaka; Watanabe, Takafumi; Miyazaki, Hiroshi; Kato, Takashi.

    In: Journal of Biochemistry, Vol. 138, No. 2, 08.2005, p. 167-175.

    Research output: Contribution to journalArticle

    Aizawa, Youichi ; Nogawa, Nami ; Kosaka, Nobuyoshi ; Maeda, Yasutaka ; Watanabe, Takafumi ; Miyazaki, Hiroshi ; Kato, Takashi. / Expression of erythropoietin receptor-like molecule in Xenopus laevis and erythrocytopenia upon administration of its recombinant soluble form. In: Journal of Biochemistry. 2005 ; Vol. 138, No. 2. pp. 167-175.
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    abstract = "The regulation of hematopoiesis in non-mammalian vertebrates is poorly understood. This is partly because the structures and effects of most hematopoietic regulators have not been identified. As a first step towards studies on the key mechanism of hematopoietic regulation among phyla as well as the diversity of organisms, we have focused on amphibian hematopoiesis. A cDNA sharing the highest degree of homology with mammalian erythropoietin (EPO) receptors, tentatively named xlEPOR, was cloned from a cDNA library of Xenopus laevis immature erythrocytes. The comparative identities of the deduced entire amino acid sequence to mammalian EPO receptors were quite low, although functional domains indispensable for erythropoietic activities were found in the molecule. Northern analysis revealed that xlEPOR were expressed in peripheral blood cells. In the peripheral blood of phenylhydrazine-treated adult Xenopus, immature erythrocytes expressing xlEPOR were identified by in situ hybridization and immunostaining with polyclonal antibodies to xlEPOR. To confirm the biological functions of this molecule, the extracellular domain of xlEPOR (i.e., soluble xlEPOR) was administered to adult Xenopus by consecutive intracardiac injection. The peripheral erythrocyte counts were decreased gradually; meanwhile, immature erythrocytes appeared in the circulation, demonstrating that xlEPOR plays a significant physiological role in erythropoiesis in Xenopus laevis.",
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