Expression of FTZ-F1α in frog testicular cells

Minoru Takase, Takeshi Nakajima, Masahisa Nakamura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Fushi tarazu transcription factor-1 (FTZ-F1), a member of a nuclear hormone receptor superfamily, is a transcriptional regulator for fushi tarazu gene expression in Drosophila (Ueda et al., '90). We have cloned a homologue (rrFTZ-F1α) of the FTZ-F1 gene of the frog Rana rugosa. The gene, in frogs, has been shown to have high expression level in the testis (Nakajima et al., 2000). In this study, the RT-PCR analysis showed that the FTZ-Flα mRNA level in adult frogs did not change throughout the year, even during hibernation. However, when immunohistological studies using the anti-rrFTZ-F1α antibody were employed to examine which testicular cells expressed this gene, Sertoli cells were found to produce rrFTZ-F1α in the two seasons - the breeding season (from March through May) and the pre-hibernating season (from October through November). Interstitial cells, however, did it in only the breeding season (from April through May). Taken together, the results suggest that the rrFTZ-F1α expression is regulated at the post-transcriptional step, and that the rrFTZ-F1α may play an important role(s) in the seasonal activities of Sertoli and interstitial cells in the frog testis.

Original languageEnglish
Pages (from-to)182-189
Number of pages8
JournalJournal of Experimental Zoology
Volume290
Issue number2
DOIs
Publication statusPublished - 2001 Jul 1
Externally publishedYes

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frogs
transcription factors
breeding season
testes
cells
genes
Sertoli cells
hormone receptors
hibernation
Drosophila
reverse transcriptase polymerase chain reaction
gene expression
antibodies

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Expression of FTZ-F1α in frog testicular cells. / Takase, Minoru; Nakajima, Takeshi; Nakamura, Masahisa.

In: Journal of Experimental Zoology, Vol. 290, No. 2, 01.07.2001, p. 182-189.

Research output: Contribution to journalArticle

Takase, M, Nakajima, T & Nakamura, M 2001, 'Expression of FTZ-F1α in frog testicular cells', Journal of Experimental Zoology, vol. 290, no. 2, pp. 182-189. https://doi.org/10.1002/jez.1048
Takase, Minoru ; Nakajima, Takeshi ; Nakamura, Masahisa. / Expression of FTZ-F1α in frog testicular cells. In: Journal of Experimental Zoology. 2001 ; Vol. 290, No. 2. pp. 182-189.
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