Expression of gibberellin 3 beta-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry.

Mami Sugano, Yuriko Nakagawa, Hiroshi Nyunoya, Teruko Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Expressions of the gibberellin biosynthesis gene were investigated in a normal upright type and a gravi-response mutant, a weeping type of Japanese flowering cherry (Prunus spachiana), that is unable to support its own weight and elongates downward. A segment of the gibberellin 3 beta-hydroxylase cDNA of Prunus spachiana (Ps3ox), which is responsible for active gibberellin synthesis, was amplified by using real-time RT-PCR. The content of Ps3ox mRNA in the weeping type was much greater than that in the upright type, while the endogenous gibberellin level was much higher in the elongating zone of the weeping type. These results suggest that the amount and distribution of synthesized gibberellin regulate secondary xylem formation, and the unbalanced distribution of gibberellin affects the gravi-response of the Prunus tree.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalBiological sciences in space = Uchū seibutsu kagaku
Volume18
Issue number4
Publication statusPublished - 2004
Externally publishedYes

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Gibberellins
Mixed Function Oxygenases
Prunus
Genes
Xylem
Real-Time Polymerase Chain Reaction
Complementary DNA
Weights and Measures
Messenger RNA

Cite this

Expression of gibberellin 3 beta-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry. / Sugano, Mami; Nakagawa, Yuriko; Nyunoya, Hiroshi; Nakamura, Teruko.

In: Biological sciences in space = Uchū seibutsu kagaku, Vol. 18, No. 4, 2004, p. 261-266.

Research output: Contribution to journalArticle

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