Expression of hybrid isomyosins in human skeletal muscle

Masanobu Wada, Tadashi Okumoto, Kyoko Toro, Kazumi Masuda, Toru Fukubayashi, Kunio Kikuchi, Shigemitsu Niihata, Shigeru Katsuta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Myosin of human skeletal muscles was analyzed by means of several electrophoretic techniques. Myosin heavy chain (HC)-IIa- and HC-IIb-based isomyosins were identified by pyrophosphate-polyacrylamide gel electrophoresis (PP-PAGE). The electrophoretic mobilities of these fast- twitch muscle isomyosins differed in the order HC-IIa triplets < HC-IIb triplets. To determine the subunit composition of myosin molecules that function in intact muscle, two-dimensional electrophoresis in which the first and second dimensions were PP-PAGE and sodium dodecyl sulfate-PAGE, respectively, was also performed. Slow-twitch muscle isomyosin contained, in addition to slow-twitch light chain (LC) and HC-I isoforms, appreciable amounts of LC-2f, HC-IIa, and HC-IIb isoforms, and fast-twitch muscle isomyosin consisted of LC-2s and HC-I isoforms as well as fast-twitch LC and HC isoforms. Without consideration of HC- and slow-twitch alkali LC heterodimers, at least 31 possible isomyosins are derived from these findings on the subunit composition of isomyosins in human skeletal muscle.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number4 40-4
Publication statusPublished - 1996 Oct
Externally publishedYes

Fingerprint

Muscle
Skeletal Muscle
Protein Isoforms
Light
Polyacrylamide Gel Electrophoresis
Muscles
Electrophoresis
Myosins
Skeletal Muscle Myosins
Myosin Heavy Chains
Slow light
Alkalies
Electrophoretic mobility
Chemical analysis
Sodium Dodecyl Sulfate
Molecules
diphosphoric acid
polyacrylamide gels

Keywords

  • hybrid
  • myosin heavy chain
  • myosin light chain

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Wada, M., Okumoto, T., Toro, K., Masuda, K., Fukubayashi, T., Kikuchi, K., ... Katsuta, S. (1996). Expression of hybrid isomyosins in human skeletal muscle. American Journal of Physiology - Cell Physiology, 271(4 40-4).

Expression of hybrid isomyosins in human skeletal muscle. / Wada, Masanobu; Okumoto, Tadashi; Toro, Kyoko; Masuda, Kazumi; Fukubayashi, Toru; Kikuchi, Kunio; Niihata, Shigemitsu; Katsuta, Shigeru.

In: American Journal of Physiology - Cell Physiology, Vol. 271, No. 4 40-4, 10.1996.

Research output: Contribution to journalArticle

Wada, M, Okumoto, T, Toro, K, Masuda, K, Fukubayashi, T, Kikuchi, K, Niihata, S & Katsuta, S 1996, 'Expression of hybrid isomyosins in human skeletal muscle', American Journal of Physiology - Cell Physiology, vol. 271, no. 4 40-4.
Wada M, Okumoto T, Toro K, Masuda K, Fukubayashi T, Kikuchi K et al. Expression of hybrid isomyosins in human skeletal muscle. American Journal of Physiology - Cell Physiology. 1996 Oct;271(4 40-4).
Wada, Masanobu ; Okumoto, Tadashi ; Toro, Kyoko ; Masuda, Kazumi ; Fukubayashi, Toru ; Kikuchi, Kunio ; Niihata, Shigemitsu ; Katsuta, Shigeru. / Expression of hybrid isomyosins in human skeletal muscle. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 271, No. 4 40-4.
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