Expression of Lhx9 isoforms in the developing gonads of Rana rugosa

Yuki Oshima, Koki Noguchi, Masahisa Nakamura

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Lhx9 is a LIM-homeodomain (HD) transcription factor. Transcripts of the Lhx9 gene are present in the urogenital ridges of mouse embryos. In Lhx9-deficient mice, a discrete gonad is not formed (Birk et al., 2000). To date, however, the role of Lhx9 in gonad formation has not been studied in animals other than chicks and mice. Thus, this study was aimed at determining whether or not Lhx9 is expressed in the developing gonads of amphibians. We first isolated cDNAs of Lhx9 and four isoforms, named Lhx9α, β, γand δ, from the frog Rana rugosa. All the isoforms lacked the last 14 amino acids of the HD (the DNA-binding motif). RT-PCR analysis revealed that Lhx9 and Lhx9α were highly expressed in the ovary, testis, brain and heart of adult frogs. However, neither Lhx9 nor any of the four isoforms was expressed in the pancreas and kidney. When their expression in the developing gonads of R. rugosa was examined by RT-PCR, transcripts of Lhx9 and Lhx9α were detected with no sexual dimorphism during sex determination. Lhx9γ and Lhx9δ were also expressed, but at a low level. In contrast, the Lhx9β transcript was hardly detected. In addition, in situ hybridization analysis showed that R. rugosa Lhx9 was expressed in somatic cells in the developing gonad and mesonephros far before sex determination. Taken together, Lhx9 and its four isoforms are probably involved in organogenesis in R. rugosa. All the isoforms may function as an endogenous dominant-negative form of Lhx9 in this species.

    Original languageEnglish
    Pages (from-to)798-802
    Number of pages5
    JournalZoological Science
    Volume24
    Issue number8
    DOIs
    Publication statusPublished - 2007 Aug

    Fingerprint

    gonads
    frogs
    mice
    reverse transcriptase polymerase chain reaction
    DNA-binding domains
    gender
    organogenesis
    somatic cells
    pancreas
    sexual dimorphism
    in situ hybridization
    amphibians
    testes
    embryo (animal)
    transcription factors
    chicks
    heart
    kidneys
    Glandirana rugosa
    brain

    Keywords

    • Frog
    • Gonad
    • Isoforms
    • Lhx9
    • Rana rugosa
    • Sex determination

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Expression of Lhx9 isoforms in the developing gonads of Rana rugosa. / Oshima, Yuki; Noguchi, Koki; Nakamura, Masahisa.

    In: Zoological Science, Vol. 24, No. 8, 08.2007, p. 798-802.

    Research output: Contribution to journalArticle

    Oshima, Yuki ; Noguchi, Koki ; Nakamura, Masahisa. / Expression of Lhx9 isoforms in the developing gonads of Rana rugosa. In: Zoological Science. 2007 ; Vol. 24, No. 8. pp. 798-802.
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    abstract = "Lhx9 is a LIM-homeodomain (HD) transcription factor. Transcripts of the Lhx9 gene are present in the urogenital ridges of mouse embryos. In Lhx9-deficient mice, a discrete gonad is not formed (Birk et al., 2000). To date, however, the role of Lhx9 in gonad formation has not been studied in animals other than chicks and mice. Thus, this study was aimed at determining whether or not Lhx9 is expressed in the developing gonads of amphibians. We first isolated cDNAs of Lhx9 and four isoforms, named Lhx9α, β, γand δ, from the frog Rana rugosa. All the isoforms lacked the last 14 amino acids of the HD (the DNA-binding motif). RT-PCR analysis revealed that Lhx9 and Lhx9α were highly expressed in the ovary, testis, brain and heart of adult frogs. However, neither Lhx9 nor any of the four isoforms was expressed in the pancreas and kidney. When their expression in the developing gonads of R. rugosa was examined by RT-PCR, transcripts of Lhx9 and Lhx9α were detected with no sexual dimorphism during sex determination. Lhx9γ and Lhx9δ were also expressed, but at a low level. In contrast, the Lhx9β transcript was hardly detected. In addition, in situ hybridization analysis showed that R. rugosa Lhx9 was expressed in somatic cells in the developing gonad and mesonephros far before sex determination. Taken together, Lhx9 and its four isoforms are probably involved in organogenesis in R. rugosa. All the isoforms may function as an endogenous dominant-negative form of Lhx9 in this species.",
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    AB - Lhx9 is a LIM-homeodomain (HD) transcription factor. Transcripts of the Lhx9 gene are present in the urogenital ridges of mouse embryos. In Lhx9-deficient mice, a discrete gonad is not formed (Birk et al., 2000). To date, however, the role of Lhx9 in gonad formation has not been studied in animals other than chicks and mice. Thus, this study was aimed at determining whether or not Lhx9 is expressed in the developing gonads of amphibians. We first isolated cDNAs of Lhx9 and four isoforms, named Lhx9α, β, γand δ, from the frog Rana rugosa. All the isoforms lacked the last 14 amino acids of the HD (the DNA-binding motif). RT-PCR analysis revealed that Lhx9 and Lhx9α were highly expressed in the ovary, testis, brain and heart of adult frogs. However, neither Lhx9 nor any of the four isoforms was expressed in the pancreas and kidney. When their expression in the developing gonads of R. rugosa was examined by RT-PCR, transcripts of Lhx9 and Lhx9α were detected with no sexual dimorphism during sex determination. Lhx9γ and Lhx9δ were also expressed, but at a low level. In contrast, the Lhx9β transcript was hardly detected. In addition, in situ hybridization analysis showed that R. rugosa Lhx9 was expressed in somatic cells in the developing gonad and mesonephros far before sex determination. Taken together, Lhx9 and its four isoforms are probably involved in organogenesis in R. rugosa. All the isoforms may function as an endogenous dominant-negative form of Lhx9 in this species.

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