Expression of liver specific genes in double layered co-culture of rat hepatocytes and human umbilical vein endothelial cells by cell sheet engineering

Y. Kurosawa*, Y. Shirai, T. Okano, A. Taniguchi

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The liver specific genes expression level in double layered co-cultured system was investigated by the comparison with the monolayer cultured Fao cells. Human umbilical vein endothelical cells (HUVEC) were seeded and cultured at 37°C on temperature-responsive culture dish grafted with PIPAAm. RNA was extracted from the double-layered cell sheets co-culture and Fao cell to which the monomer was cultured. It was observed that the lever specific genes expressions of APO A-1 and APO C-III were related to the lipid metabolism, increased by double layered co-culture system compared with monolayer cultured Fao cells.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004 Dec 1
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period04/5/1704/5/21

ASJC Scopus subject areas

  • Engineering(all)

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