Expression of liver specific genes in double layered co-culture of rat hepatocytes and human umbilical vein endothelial cells by cell sheet engineering

Y. Kurosawa, Y. Shirai, T. Okano, Akiyoshi Taniguchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The liver specific genes expression level in double layered co-cultured system was investigated by the comparison with the monolayer cultured Fao cells. Human umbilical vein endothelical cells (HUVEC) were seeded and cultured at 37°C on temperature-responsive culture dish grafted with PIPAAm. RNA was extracted from the double-layered cell sheets co-culture and Fao cell to which the monomer was cultured. It was observed that the lever specific genes expressions of APO A-1 and APO C-III were related to the lipid metabolism, increased by double layered co-culture system compared with monolayer cultured Fao cells.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1857
Number of pages1
Publication statusPublished - 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04/5/1704/5/21

Fingerprint

Endothelial cells
Cell culture
Gene expression
Liver
Rats
Monolayers
Genes
RNA
Monomers
Temperature
Hepatocytes
Lipid Metabolism

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Expression of liver specific genes in double layered co-culture of rat hepatocytes and human umbilical vein endothelial cells by cell sheet engineering. / Kurosawa, Y.; Shirai, Y.; Okano, T.; Taniguchi, Akiyoshi.

Transactions - 7th World Biomaterials Congress. 2004. p. 1857.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kurosawa, Y, Shirai, Y, Okano, T & Taniguchi, A 2004, Expression of liver specific genes in double layered co-culture of rat hepatocytes and human umbilical vein endothelial cells by cell sheet engineering. in Transactions - 7th World Biomaterials Congress. pp. 1857, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 04/5/17.
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