Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Zhonggang Feng, Rie Takahashi, Takao Nakamura, Daisuke Sato, Nobuyuki Shirasawa, Ayako Nakayama, Sakura Kurashige, Tadashi Kosawada, Tatsuo Kitajima, Mitsuo Umezu

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.

    Original languageEnglish
    Pages (from-to)700-706
    Number of pages7
    JournalIn Vitro Cellular and Developmental Biology - Animal
    Volume50
    Issue number8
    DOIs
    Publication statusPublished - 2014 Sep 1

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    MicroRNAs
    Cardiac Myocytes
    Proteins
    Messenger RNA
    Connexin 43
    Myofibrils
    Cadherins
    Wistar Rats
    Cultured Cells
    Stem Cells
    Observation

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes. / Feng, Zhonggang; Takahashi, Rie; Nakamura, Takao; Sato, Daisuke; Shirasawa, Nobuyuki; Nakayama, Ayako; Kurashige, Sakura; Kosawada, Tadashi; Kitajima, Tatsuo; Umezu, Mitsuo.

    In: In Vitro Cellular and Developmental Biology - Animal, Vol. 50, No. 8, 01.09.2014, p. 700-706.

    Research output: Contribution to journalArticle

    Feng, Z, Takahashi, R, Nakamura, T, Sato, D, Shirasawa, N, Nakayama, A, Kurashige, S, Kosawada, T, Kitajima, T & Umezu, M 2014, 'Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes', In Vitro Cellular and Developmental Biology - Animal, vol. 50, no. 8, pp. 700-706. https://doi.org/10.1007/s11626-014-9755-0
    Feng, Zhonggang ; Takahashi, Rie ; Nakamura, Takao ; Sato, Daisuke ; Shirasawa, Nobuyuki ; Nakayama, Ayako ; Kurashige, Sakura ; Kosawada, Tadashi ; Kitajima, Tatsuo ; Umezu, Mitsuo. / Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes. In: In Vitro Cellular and Developmental Biology - Animal. 2014 ; Vol. 50, No. 8. pp. 700-706.
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    AU - Shirasawa, Nobuyuki

    AU - Nakayama, Ayako

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