TY - JOUR
T1 - Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes
AU - Feng, Zhonggang
AU - Takahashi, Rie
AU - Nakamura, Takao
AU - Sato, Daisuke
AU - Shirasawa, Nobuyuki
AU - Nakayama, Ayako
AU - Kurashige, Sakura
AU - Kosawada, Tadashi
AU - Kitajima, Tatsuo
AU - Umezu, Mitsuo
N1 - Funding Information:
Acknowledgment This study is supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (25350521).
PY - 2014/9
Y1 - 2014/9
N2 - In this study, we investigated the expression of the pathway, SRF–microRNA-1/microRNA-133a–Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, Ncadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF–microRNA- 1/microRNA-133a–Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.
AB - In this study, we investigated the expression of the pathway, SRF–microRNA-1/microRNA-133a–Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, Ncadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF–microRNA- 1/microRNA-133a–Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.
KW - Cell culture
KW - Embryonic cardiomyocytes
KW - Hand2
KW - Immaturity
KW - miRNA-1
KW - miRNA-133a
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UR - http://www.scopus.com/inward/citedby.url?scp=84932106094&partnerID=8YFLogxK
U2 - 10.1007/s11626-014-9755-0
DO - 10.1007/s11626-014-9755-0
M3 - Article
C2 - 24789724
AN - SCOPUS:84932106094
VL - 50
SP - 700
EP - 706
JO - In Vitro
JF - In Vitro
SN - 0073-5655
IS - 8
ER -