Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Zhonggang Feng, Rie Takahashi, Takao Nakamura, Daisuke Sato, Nobuyuki Shirasawa, Ayako Nakayama, Sakura Kurashige, Tadashi Kosawada, Tatsuo Kitajima, Mitsuo Umezu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this study, we investigated the expression of the pathway, SRF–microRNA-1/microRNA-133a–Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, Ncadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF–microRNA- 1/microRNA-133a–Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.

Original languageEnglish
Pages (from-to)700-706
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume50
Issue number8
DOIs
Publication statusPublished - 2014 Sep

Keywords

  • Cell culture
  • Embryonic cardiomyocytes
  • Hand2
  • Immaturity
  • miRNA-1
  • miRNA-133a

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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