Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Zhonggang Feng; Rie Takahashi; Takao Nakamura; Daisuke Sato; Nobuyuki Shirasawa; Ayako Nakayama; Sakura Kurashige; Tadashi Kosawada; Tatsuo Kitajima; Mitsuo Umezu

doi:10.1007/s11626-014-9755-0

Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

Zhonggang Feng, Rie Takahashi, Takao Nakamura, Daisuke Sato, Nobuyuki Shirasawa, Ayako Nakayama, Sakura Kurashige, Tadashi Kosawada, Tatsuo Kitajima, Mitsuo Umezu

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.

Original language	English
Pages (from-to)	700-706
Number of pages	7
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	50
Issue number	8
DOIs	https://doi.org/10.1007/s11626-014-9755-0
Publication status	Published - 2014 Sep 1

ASJC Scopus subject areas

Medicine(all)

Access to Document

10.1007/s11626-014-9755-0

Fingerprint Dive into the research topics of 'Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes'. Together they form a unique fingerprint.

Cite this

Feng, Z., Takahashi, R., Nakamura, T., Sato, D., Shirasawa, N., Nakayama, A., Kurashige, S., Kosawada, T., Kitajima, T., & Umezu, M. (2014). Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes. In Vitro Cellular and Developmental Biology - Animal, 50(8), 700-706. https://doi.org/10.1007/s11626-014-9755-0

Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes. / Feng, Zhonggang; Takahashi, Rie; Nakamura, Takao; Sato, Daisuke; Shirasawa, Nobuyuki; Nakayama, Ayako; Kurashige, Sakura; Kosawada, Tadashi; Kitajima, Tatsuo; Umezu, Mitsuo.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 50, No. 8, 01.09.2014, p. 700-706.

Research output: Contribution to journal › Article

Feng, Zhonggang ; Takahashi, Rie ; Nakamura, Takao ; Sato, Daisuke ; Shirasawa, Nobuyuki ; Nakayama, Ayako ; Kurashige, Sakura ; Kosawada, Tadashi ; Kitajima, Tatsuo ; Umezu, Mitsuo. / Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes. In: In Vitro Cellular and Developmental Biology - Animal. 2014 ; Vol. 50, No. 8. pp. 700-706.

@article{c20bf21738cf4a3389e4b76e55bc3a06,

title = "Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes",

abstract = "In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells. ",

author = "Zhonggang Feng and Rie Takahashi and Takao Nakamura and Daisuke Sato and Nobuyuki Shirasawa and Ayako Nakayama and Sakura Kurashige and Tadashi Kosawada and Tatsuo Kitajima and Mitsuo Umezu",

year = "2014",

month = sep,

day = "1",

doi = "10.1007/s11626-014-9755-0",

language = "English",

volume = "50",

pages = "700--706",

journal = "In Vitro Cellular and Developmental Biology - Animal",

issn = "1071-2690",

publisher = "Springer New York",

number = "8",

}

TY - JOUR

T1 - Expression of microRNA-1, microRNA-133a and Hand2 protein in cultured embryonic rat cardiomyocytes

AU - Feng, Zhonggang

AU - Takahashi, Rie

AU - Nakamura, Takao

AU - Sato, Daisuke

AU - Shirasawa, Nobuyuki

AU - Nakayama, Ayako

AU - Kurashige, Sakura

AU - Kosawada, Tadashi

AU - Kitajima, Tatsuo

AU - Umezu, Mitsuo

PY - 2014/9/1

Y1 - 2014/9/1

N2 - In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.

AB - In this study, we investigated the expression of the pathway, SRF-microRNA-1/microRNA-133a-Hand2, in the Wistar rat embryonic ventricular cardiomyocytes under conventional monolayer culture. The morphological observation of the cultured cardiomyocytes and the mRNA expression levels of three vital constituent proteins, MLC-2v, N-cadherin, and connexin43, demonstrated the immaturity of these cultured cells, which was featured by less myofibril density, immature sarcomeric structure, and significantly lower mRNA expression of the three constituent proteins than those in neonatal ventricular samples. More importantly, results in this study suggest that the change of SRF-microRNA-1/microRNA-133a-Hand2 pathway results into the attenuation of the Hand2 repression in cultured cardiomyocytes. These outcomes are valuable to understand the cellular state as embryonic cardiomyocytes to be in vitro model and might be useful for the assessment of engineered cardiac tissue and cardiac differentiation of stem cells.

UR - http://www.scopus.com/inward/record.url?scp=84932106094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84932106094&partnerID=8YFLogxK

U2 - 10.1007/s11626-014-9755-0

DO - 10.1007/s11626-014-9755-0

M3 - Article

C2 - 24789724

AN - SCOPUS:84932106094

VL - 50

SP - 700

EP - 706

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

IS - 8

ER -