Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells

Mitsuki Nozaki, Shogo Haraguchi, Takuro Miyazaki, Daichi Shigeta, Noriko Kano, Xiao Feng Lei, Joo Ri Kim-Kaneyama, Hiroyuki Minakata, Akira Miyazaki, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

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    Abstract

    The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids. However, a preliminary study suggested that COS-7 cells metabolize [3H]testosterone to [3H]androstenedione. These results suggest that COS-7 cells are able to metabolize steroids. Therefore, the present study investigated the expression of steroidogenic enzymes and the metabolism of steroids in COS-7 cells. RT-PCR analyses demonstrated the expressions of several kinds of steroidogenic enzymes, such as cytochrome P450 side-chain cleavage enzyme, 3β-hydroxysteroid dehydrogenase/Δ54 isomerase, cytochrome P450 7α-hydroxylase, cytochrome P450 17α-hydroxylase/17,20-lyase, 17β-hydroxysteroid dehydrogenase, 5α-reductase, cytochrome P450 21-hydroxylase, cytochrome P450 11β-hydroxylase, and cytochrome P450 aromatase in COS-7 cells. In addition, steroidogenic enzymes 3β-HSD, P4507α, 5α-reductase, P450c17, P450c21, P450c11β, and 17β-HSD actively metabolized various steroids in cultured COS-7 cells. Finally, we demonstrated that 17β-HSD activity toward androstenedione formation was greater than other steroidogenic enzyme activities. Our results provide new evidence that COS-7 cells express a series of steroidogenic enzyme mRNAs and actively metabolize a variety of steroids.

    Original languageEnglish
    Article number2167
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    Publication statusPublished - 2018 Dec 1

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    COS Cells
    Steroids
    Cytochrome P-450 Enzyme System
    Enzymes
    Mixed Function Oxygenases
    Androstenedione
    3-Hydroxysteroid Dehydrogenases
    Cholesterol 7-alpha-Hydroxylase
    Steroid 17-alpha-Hydroxylase
    Steroid 21-Hydroxylase
    Kidney
    Isomerases
    NADPH-Ferrihemoprotein Reductase
    Aromatase
    Transfection
    Testosterone
    Cultured Cells
    Oxidoreductases
    Polymerase Chain Reaction
    Messenger RNA

    ASJC Scopus subject areas

    • General

    Cite this

    Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells. / Nozaki, Mitsuki; Haraguchi, Shogo; Miyazaki, Takuro; Shigeta, Daichi; Kano, Noriko; Lei, Xiao Feng; Kim-Kaneyama, Joo Ri; Minakata, Hiroyuki; Miyazaki, Akira; Tsutsui, Kazuyoshi.

    In: Scientific Reports, Vol. 8, No. 1, 2167, 01.12.2018.

    Research output: Contribution to journalArticle

    Nozaki, M, Haraguchi, S, Miyazaki, T, Shigeta, D, Kano, N, Lei, XF, Kim-Kaneyama, JR, Minakata, H, Miyazaki, A & Tsutsui, K 2018, 'Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells', Scientific Reports, vol. 8, no. 1, 2167. https://doi.org/10.1038/s41598-018-20226-2
    Nozaki, Mitsuki ; Haraguchi, Shogo ; Miyazaki, Takuro ; Shigeta, Daichi ; Kano, Noriko ; Lei, Xiao Feng ; Kim-Kaneyama, Joo Ri ; Minakata, Hiroyuki ; Miyazaki, Akira ; Tsutsui, Kazuyoshi. / Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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    AU - Kano, Noriko

    AU - Lei, Xiao Feng

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    AU - Miyazaki, Akira

    AU - Tsutsui, Kazuyoshi

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