Expression of steroidogenic factor-1 in frog embryo and developing gonad

K. I. Kawano, S. Furusawa, H. Matsuda, M. Takase, M. Nakamura

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Steroidogenic factor-1 (SF-1), originally identified as an orphan nuclear receptor that regulates expression of genes encoding cytochrome P-450 steroid hydroxylases, is an essential transcriptional factor for adrenal and gonadal development in mammals. Since sex steroid hormones have been shown to play important roles in the sex determination of frogs, it is of interest to know how SF-1 gene expression is regulated during the sexual development of this organism. A previous study isolated the cDNA of the frog Rana rugosa SF-1 (rrSF-1) and found sexual differences in its gene expression in adult frogs; positive in testis and negative in ovary (Kawano et al., 1998). This study examined rrSF-1 gene expression in frog embryos and developing and mature gonads by immunohistochemical staining using anti-rrSF-1 protein antibody for protein localization and by in situ hybridization analysis for mRNA transcription. The results obtained in this study indicated that cells expressing SF-1 that originate in the mesoderm and endoderm probably migrate into the developing gonad via the dorsal mesentery, genital ridge, and mesorchium or mesovarium. Thus, SF-1 may play an important role in gonadal development in amphibians.

    Original languageEnglish
    Pages (from-to)13-22
    Number of pages10
    JournalGeneral and Comparative Endocrinology
    Volume123
    Issue number1
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Steroidogenic Factor 1
    Gonads
    Anura
    gonads
    frogs
    embryo (animal)
    Ranidae
    Embryonic Structures
    gene expression
    Gene Expression
    mesentery
    Steroid Hydroxylases
    sex hormones
    sexual development
    Orphan Nuclear Receptors
    steroid hormones
    Endoderm
    Sexual Development
    cytochrome P-450
    Mesentery

    Keywords

    • Embryo
    • Frog
    • Gonad
    • Immunohistology
    • In situ hybridization
    • SF-1
    • WT-1

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Expression of steroidogenic factor-1 in frog embryo and developing gonad. / Kawano, K. I.; Furusawa, S.; Matsuda, H.; Takase, M.; Nakamura, M.

    In: General and Comparative Endocrinology, Vol. 123, No. 1, 2001, p. 13-22.

    Research output: Contribution to journalArticle

    Kawano, KI, Furusawa, S, Matsuda, H, Takase, M & Nakamura, M 2001, 'Expression of steroidogenic factor-1 in frog embryo and developing gonad', General and Comparative Endocrinology, vol. 123, no. 1, pp. 13-22. https://doi.org/10.1006/gcen.2000.7584
    Kawano, K. I. ; Furusawa, S. ; Matsuda, H. ; Takase, M. ; Nakamura, M. / Expression of steroidogenic factor-1 in frog embryo and developing gonad. In: General and Comparative Endocrinology. 2001 ; Vol. 123, No. 1. pp. 13-22.
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