Expression of the melatonin receptor Mel1c in neural tissues of the reef fish Siganus guttatus

Yong Ju Park, Ji Gweon Park, Hyung Bok Jeong, Yuki Takeuchi, Se Jae Kim, Young Don Lee, Akihiro Takemura

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The golden rabbitfish, Siganus guttatus, is a reef fish exhibiting a restricted lunar-related rhythm in behavior and reproduction. Here, to understand the circadian rhythm of this lunar-synchronized spawner, a melatonin receptor subtype-Mel1c-was cloned. The full-length Mel1c melatonin receptor cDNA comprised 1747 bp with a single open reading frame (1062 bp) that encodes a 353-amino acid protein, which included 7 presumed transmembrane domains. Real-time PCR revealed high Mel1c mRNA expression in the retina and brain but not in the peripheral tissues. When the fish were reared under light/dark (LD 12:12) conditions, Mel1c mRNA in the retina and brain was expressed with daily variations and increased during nighttime. Similar variations were noted under constant conditions, suggesting that Mel1c mRNA expression is regulated by the circadian clock system. Daily variations of Mel1c mRNA expression with a peak at zeitgeber time (ZT) 12 were observed in the cultured pineal gland under LD 12:12. Exposure of the cultured pineal gland to light at ZT17 resulted in a decrease in Mel1c mRNA expression. When light was obstructed at ZT5, the opposite effect was obtained. These results suggest that light exerts certain effects on Mel1c mRNA expression directly or indirectly through melatonin actions.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume147
Issue number1
DOIs
Publication statusPublished - 2007 May
Externally publishedYes

Fingerprint

Melatonin Receptors
Reefs
Fish
Fishes
Tissue
Messenger RNA
Light
Pineal Gland
Retina
Brain
Circadian Clocks
Melatonin
Circadian Rhythm
Open Reading Frames
Reproduction
Real-Time Polymerase Chain Reaction
Clocks
Complementary DNA
Amino Acids

Keywords

  • Circadian rhythm
  • Daily variations
  • Golden rabbitfish
  • Mel
  • Melatonin
  • Melatonin receptor
  • Pineal gland
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Expression of the melatonin receptor Mel1c in neural tissues of the reef fish Siganus guttatus. / Park, Yong Ju; Park, Ji Gweon; Jeong, Hyung Bok; Takeuchi, Yuki; Kim, Se Jae; Lee, Young Don; Takemura, Akihiro.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 147, No. 1, 05.2007, p. 103-111.

Research output: Contribution to journalArticle

Park, Yong Ju ; Park, Ji Gweon ; Jeong, Hyung Bok ; Takeuchi, Yuki ; Kim, Se Jae ; Lee, Young Don ; Takemura, Akihiro. / Expression of the melatonin receptor Mel1c in neural tissues of the reef fish Siganus guttatus. In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology. 2007 ; Vol. 147, No. 1. pp. 103-111.
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