Expression of three nonallelic genes coding calmodulin exhibits similar localization on the central nervous system of adult rats

H. Ikeshima, S. Yuasa, K. Matsuo, K. Kawamura, J. Hata, T. Takano

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

By Northern blot analysis with the digoxigenin-labeled antisense RNA probes of the noncoding regions, the transcripts of three calmodulin (CaM) genes, CaMI, CaMII, and CaMIII, were separately detected in 12 different tissues of adult Wistar albino rats, without any cross-hybridization. The mRNAs of all three CaM genes were abundant in the central nervous system (CNS) as well as in the testis, although ubiquitous expression was detected at low levels in the other tissues. There were subtle but significant differences in the tissue-specific distribution of the three CaM gene RNAs. By in situ hybridization, strong hybridization of the three CaM gene probes was observed in common in large projection neurons of the CNS: the hippocampal pyramidal cells, the cerebellar Purkinje cells, and the large neurons of the cerebral neocortex, the pyriform cortex, the mesencephalon, the pons, and the spinal cord. The expression of the three CaM genes was at lower levels in small interneurons of the CNS. These profiles of expression were almost the same among the three CaM genes. Thus, all three CaM genes were coordinately expressed in neurons of the adult rat CNS. Certain regulatory mechanisms of the three CaM genes seemed to mediate similar tissue- and cell type-specific expression in the CNS.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalJournal of Neuroscience Research
Volume36
Issue number1
Publication statusPublished - 1993
Externally publishedYes

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Calmodulin
Central Nervous System
Genes
Neurons
RNA Probes
Digoxigenin
Antisense RNA
Pons
Pyramidal Cells
Purkinje Cells
Neocortex
Interneurons
Tissue Distribution
Mesencephalon
Northern Blotting
In Situ Hybridization
Testis
Wistar Rats
Spinal Cord
RNA

Keywords

  • brain
  • in situ hybridization
  • mRNA
  • Northern blot analysis
  • spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of three nonallelic genes coding calmodulin exhibits similar localization on the central nervous system of adult rats. / Ikeshima, H.; Yuasa, S.; Matsuo, K.; Kawamura, K.; Hata, J.; Takano, T.

In: Journal of Neuroscience Research, Vol. 36, No. 1, 1993, p. 111-119.

Research output: Contribution to journalArticle

Ikeshima, H, Yuasa, S, Matsuo, K, Kawamura, K, Hata, J & Takano, T 1993, 'Expression of three nonallelic genes coding calmodulin exhibits similar localization on the central nervous system of adult rats', Journal of Neuroscience Research, vol. 36, no. 1, pp. 111-119.
Ikeshima, H. ; Yuasa, S. ; Matsuo, K. ; Kawamura, K. ; Hata, J. ; Takano, T. / Expression of three nonallelic genes coding calmodulin exhibits similar localization on the central nervous system of adult rats. In: Journal of Neuroscience Research. 1993 ; Vol. 36, No. 1. pp. 111-119.
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