Expression profiles of inka2 in the murine nervous system

Yumi Iwasaki, Takahito Yumoto, Shinichi Sakakibara

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Dynamic rearrangement of the actin cytoskeleton impacts many cellular characteristics in both the developing and adult central nervous systems (CNS), including the migration and adhesion of highly motile neural progenitor cells, axon guidance of immature neurons, and reconstruction of synaptic structures in the adult brain. Inka1, a known regulator of actin cytoskeleton reconstruction, is predominantly expressed by the neural crest cell lineage and regulates the migration and differentiation of these cells. In the present study, we identified a novel gene, designated as inka2, which is related to inka1. Inka2/fam212b is an evolutionarily conserved gene found in different vertebrate species and constitutes a novel gene family together with inka1. Northern blot analysis showed that inka2 mRNA was highly enriched in the nervous system. The spatiotemporal propagation cell profiles of those cells that expressed inka2 transcripts were compatible with those of Olig2-positive oligodendrocyte progenitor cells, which originate in the ventral ventricular zone during embryogenesis. Intense expression of inka2 was also noted in the proliferative neuronal progenitors in the developing cerebellum. On the other hand, immature newborn neurons in the embryonic brain showed no expression of inka2, except for the cells residing in the marginal zone of the embryonic telencephalon, which is known to contain transient cells including the non-subplate pioneer neurons and Cajal-Retzius cells. As brain development proceeds during the postnatal stage, inka2 expression emerged in some populations of immature neurons, including the neocortical pyramidal neurons, hippocampal pyramidal neurons, and granule cells migrating in the cerebellar cortex. In the adult brain, the expression of inka2 was interestingly confined in terminally differentiated neurons in the restricted forebrain regions. Taken together, as a novel regulator of actin cytoskeletons in the CNS, inka2 may be involved in multiple actin-driven processes, including cell migration and establishment of neuronal polarity.

    Original languageEnglish
    Pages (from-to)83-97
    Number of pages15
    JournalGene Expression Patterns
    Volume19
    Issue number1-2
    DOIs
    Publication statusPublished - 2015 Sep 1

    Fingerprint

    Nervous System
    Neurons
    Actin Cytoskeleton
    Pyramidal Cells
    Brain
    Cell Movement
    Stem Cells
    Central Nervous System
    Genes
    Telencephalon
    Cerebellar Cortex
    Neural Crest
    Oligodendroglia
    Cell Lineage
    Prosencephalon
    Northern Blotting
    Cerebellum
    Embryonic Development
    Vertebrates
    Actins

    Keywords

    • Central nervous system
    • Fam212b
    • In situ hybridization
    • Inka1
    • Inka2
    • Mouse
    • Neural progenitor cell
    • Oligodendrocyte progenitor cells
    • Telencephalon

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Developmental Biology

    Cite this

    Expression profiles of inka2 in the murine nervous system. / Iwasaki, Yumi; Yumoto, Takahito; Sakakibara, Shinichi.

    In: Gene Expression Patterns, Vol. 19, No. 1-2, 01.09.2015, p. 83-97.

    Research output: Contribution to journalArticle

    Iwasaki, Yumi ; Yumoto, Takahito ; Sakakibara, Shinichi. / Expression profiles of inka2 in the murine nervous system. In: Gene Expression Patterns. 2015 ; Vol. 19, No. 1-2. pp. 83-97.
    @article{7d28c65aaf0e48569a3d787eaf604823,
    title = "Expression profiles of inka2 in the murine nervous system",
    abstract = "Dynamic rearrangement of the actin cytoskeleton impacts many cellular characteristics in both the developing and adult central nervous systems (CNS), including the migration and adhesion of highly motile neural progenitor cells, axon guidance of immature neurons, and reconstruction of synaptic structures in the adult brain. Inka1, a known regulator of actin cytoskeleton reconstruction, is predominantly expressed by the neural crest cell lineage and regulates the migration and differentiation of these cells. In the present study, we identified a novel gene, designated as inka2, which is related to inka1. Inka2/fam212b is an evolutionarily conserved gene found in different vertebrate species and constitutes a novel gene family together with inka1. Northern blot analysis showed that inka2 mRNA was highly enriched in the nervous system. The spatiotemporal propagation cell profiles of those cells that expressed inka2 transcripts were compatible with those of Olig2-positive oligodendrocyte progenitor cells, which originate in the ventral ventricular zone during embryogenesis. Intense expression of inka2 was also noted in the proliferative neuronal progenitors in the developing cerebellum. On the other hand, immature newborn neurons in the embryonic brain showed no expression of inka2, except for the cells residing in the marginal zone of the embryonic telencephalon, which is known to contain transient cells including the non-subplate pioneer neurons and Cajal-Retzius cells. As brain development proceeds during the postnatal stage, inka2 expression emerged in some populations of immature neurons, including the neocortical pyramidal neurons, hippocampal pyramidal neurons, and granule cells migrating in the cerebellar cortex. In the adult brain, the expression of inka2 was interestingly confined in terminally differentiated neurons in the restricted forebrain regions. Taken together, as a novel regulator of actin cytoskeletons in the CNS, inka2 may be involved in multiple actin-driven processes, including cell migration and establishment of neuronal polarity.",
    keywords = "Central nervous system, Fam212b, In situ hybridization, Inka1, Inka2, Mouse, Neural progenitor cell, Oligodendrocyte progenitor cells, Telencephalon",
    author = "Yumi Iwasaki and Takahito Yumoto and Shinichi Sakakibara",
    year = "2015",
    month = "9",
    day = "1",
    doi = "10.1016/j.gep.2015.08.002",
    language = "English",
    volume = "19",
    pages = "83--97",
    journal = "Brain research. Gene expression patterns",
    issn = "1567-133X",
    publisher = "Elsevier",
    number = "1-2",

    }

    TY - JOUR

    T1 - Expression profiles of inka2 in the murine nervous system

    AU - Iwasaki, Yumi

    AU - Yumoto, Takahito

    AU - Sakakibara, Shinichi

    PY - 2015/9/1

    Y1 - 2015/9/1

    N2 - Dynamic rearrangement of the actin cytoskeleton impacts many cellular characteristics in both the developing and adult central nervous systems (CNS), including the migration and adhesion of highly motile neural progenitor cells, axon guidance of immature neurons, and reconstruction of synaptic structures in the adult brain. Inka1, a known regulator of actin cytoskeleton reconstruction, is predominantly expressed by the neural crest cell lineage and regulates the migration and differentiation of these cells. In the present study, we identified a novel gene, designated as inka2, which is related to inka1. Inka2/fam212b is an evolutionarily conserved gene found in different vertebrate species and constitutes a novel gene family together with inka1. Northern blot analysis showed that inka2 mRNA was highly enriched in the nervous system. The spatiotemporal propagation cell profiles of those cells that expressed inka2 transcripts were compatible with those of Olig2-positive oligodendrocyte progenitor cells, which originate in the ventral ventricular zone during embryogenesis. Intense expression of inka2 was also noted in the proliferative neuronal progenitors in the developing cerebellum. On the other hand, immature newborn neurons in the embryonic brain showed no expression of inka2, except for the cells residing in the marginal zone of the embryonic telencephalon, which is known to contain transient cells including the non-subplate pioneer neurons and Cajal-Retzius cells. As brain development proceeds during the postnatal stage, inka2 expression emerged in some populations of immature neurons, including the neocortical pyramidal neurons, hippocampal pyramidal neurons, and granule cells migrating in the cerebellar cortex. In the adult brain, the expression of inka2 was interestingly confined in terminally differentiated neurons in the restricted forebrain regions. Taken together, as a novel regulator of actin cytoskeletons in the CNS, inka2 may be involved in multiple actin-driven processes, including cell migration and establishment of neuronal polarity.

    AB - Dynamic rearrangement of the actin cytoskeleton impacts many cellular characteristics in both the developing and adult central nervous systems (CNS), including the migration and adhesion of highly motile neural progenitor cells, axon guidance of immature neurons, and reconstruction of synaptic structures in the adult brain. Inka1, a known regulator of actin cytoskeleton reconstruction, is predominantly expressed by the neural crest cell lineage and regulates the migration and differentiation of these cells. In the present study, we identified a novel gene, designated as inka2, which is related to inka1. Inka2/fam212b is an evolutionarily conserved gene found in different vertebrate species and constitutes a novel gene family together with inka1. Northern blot analysis showed that inka2 mRNA was highly enriched in the nervous system. The spatiotemporal propagation cell profiles of those cells that expressed inka2 transcripts were compatible with those of Olig2-positive oligodendrocyte progenitor cells, which originate in the ventral ventricular zone during embryogenesis. Intense expression of inka2 was also noted in the proliferative neuronal progenitors in the developing cerebellum. On the other hand, immature newborn neurons in the embryonic brain showed no expression of inka2, except for the cells residing in the marginal zone of the embryonic telencephalon, which is known to contain transient cells including the non-subplate pioneer neurons and Cajal-Retzius cells. As brain development proceeds during the postnatal stage, inka2 expression emerged in some populations of immature neurons, including the neocortical pyramidal neurons, hippocampal pyramidal neurons, and granule cells migrating in the cerebellar cortex. In the adult brain, the expression of inka2 was interestingly confined in terminally differentiated neurons in the restricted forebrain regions. Taken together, as a novel regulator of actin cytoskeletons in the CNS, inka2 may be involved in multiple actin-driven processes, including cell migration and establishment of neuronal polarity.

    KW - Central nervous system

    KW - Fam212b

    KW - In situ hybridization

    KW - Inka1

    KW - Inka2

    KW - Mouse

    KW - Neural progenitor cell

    KW - Oligodendrocyte progenitor cells

    KW - Telencephalon

    UR - http://www.scopus.com/inward/record.url?scp=84947865679&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84947865679&partnerID=8YFLogxK

    U2 - 10.1016/j.gep.2015.08.002

    DO - 10.1016/j.gep.2015.08.002

    M3 - Article

    C2 - 26292052

    AN - SCOPUS:84947865679

    VL - 19

    SP - 83

    EP - 97

    JO - Brain research. Gene expression patterns

    JF - Brain research. Gene expression patterns

    SN - 1567-133X

    IS - 1-2

    ER -