Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene

Makoto Saito; Yukiko Kato; Emi Ito; Jiro Fujimoto; Kosuke Ishikawa; Ayano Doi; Kentaro Kumazawa; Atsuka Matsui; Shiori Takebe; Takaomi Ishida; Sakura Azuma; Hiromi Mochizuki; Yoshifumi Kawamura; Yuka Yanagisawa; Reiko Honma; Jun Ichi Imai; Hirokazu Ohbayashi; Naoki Goshima; Kentaro Semba; Shinya Watanabe

doi:10.1016/j.febslet.2012.05.003

Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene

Makoto Saito, Yukiko Kato, Emi Ito, Jiro Fujimoto, Kosuke Ishikawa, Ayano Doi, Kentaro Kumazawa, Atsuka Matsui, Shiori Takebe, Takaomi Ishida, Sakura Azuma, Hiromi Mochizuki, Yoshifumi Kawamura, Yuka Yanagisawa, Reiko Honma, Jun Ichi Imai, Hirokazu Ohbayashi, Naoki Goshima, Kentaro Semba^*, Shinya Watanabe

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain "driver" genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12-21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.

Original language	English
Pages (from-to)	1708-1714
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2012.05.003
Publication status	Published - 2012 Jun 12

Keywords

ERBB2
Expression screening
GRB7
Gene amplification
Tumorigenesis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2012.05.003

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Saito, M., Kato, Y., Ito, E., Fujimoto, J., Ishikawa, K., Doi, A., Kumazawa, K., Matsui, A., Takebe, S., Ishida, T., Azuma, S., Mochizuki, H., Kawamura, Y., Yanagisawa, Y., Honma, R., Imai, J. I., Ohbayashi, H., Goshima, N., Semba, K., & Watanabe, S. (2012). Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene. FEBS Letters, 586(12), 1708-1714. https://doi.org/10.1016/j.febslet.2012.05.003

Saito, M, Kato, Y, Ito, E, Fujimoto, J, Ishikawa, K, Doi, A, Kumazawa, K, Matsui, A, Takebe, S, Ishida, T, Azuma, S, Mochizuki, H, Kawamura, Y, Yanagisawa, Y, Honma, R, Imai, JI, Ohbayashi, H, Goshima, N, Semba, K & Watanabe, S 2012, 'Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene', FEBS Letters, vol. 586, no. 12, pp. 1708-1714. https://doi.org/10.1016/j.febslet.2012.05.003

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title = "Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene",

abstract = "Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain {"}driver{"} genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12-21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.",

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author = "Makoto Saito and Yukiko Kato and Emi Ito and Jiro Fujimoto and Kosuke Ishikawa and Ayano Doi and Kentaro Kumazawa and Atsuka Matsui and Shiori Takebe and Takaomi Ishida and Sakura Azuma and Hiromi Mochizuki and Yoshifumi Kawamura and Yuka Yanagisawa and Reiko Honma and Imai, {Jun Ichi} and Hirokazu Ohbayashi and Naoki Goshima and Kentaro Semba and Shinya Watanabe",

note = "Funding Information: We thank Kumiko Semba for her secretarial assistance. This research was partially supported by JSPS KAKENHI 23241064 and a grant for translational research programs from New Energy and Industrial Technology Development Organization (NEDO). ",

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AU - Saito, Makoto

AU - Kato, Yukiko

AU - Ito, Emi

AU - Fujimoto, Jiro

AU - Ishikawa, Kosuke

AU - Doi, Ayano

AU - Kumazawa, Kentaro

AU - Matsui, Atsuka

AU - Takebe, Shiori

AU - Ishida, Takaomi

AU - Azuma, Sakura

AU - Mochizuki, Hiromi

AU - Kawamura, Yoshifumi

AU - Yanagisawa, Yuka

AU - Honma, Reiko

AU - Imai, Jun Ichi

AU - Ohbayashi, Hirokazu

AU - Goshima, Naoki

AU - Semba, Kentaro

AU - Watanabe, Shinya

N1 - Funding Information: We thank Kumiko Semba for her secretarial assistance. This research was partially supported by JSPS KAKENHI 23241064 and a grant for translational research programs from New Energy and Industrial Technology Development Organization (NEDO).

PY - 2012/6/12

Y1 - 2012/6/12

N2 - Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain "driver" genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12-21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.

AB - Gene amplification is a major genetic alteration in human cancers. Amplicons, amplified genomic regions, are believed to contain "driver" genes responsible for tumorigenesis. However, the significance of co-amplified genes has not been extensively studied. We have established an integrated analysis system of amplicons using retrovirus-mediated gene transfer coupled with a human full-length cDNA set. Applying this system to 17q12-21 amplicon observed in breast cancer, we identified GRB7 as a context-dependent oncogene, which modulates the ERBB2 signaling pathway through enhanced phosphorylation of ERBB2 and Akt. Our work provides an insight into the biological significance of gene amplification in human cancers.

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KW - Tumorigenesis

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Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene

Abstract

Keywords

ASJC Scopus subject areas

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