Expressions of tight junction proteins occludin and claudin-1 Are under the circadian control in the mouse large intestine

Implications in intestinal permeability and susceptibility to colitis

Kyoko Oh-oka, Hiroshi Kono, Kayoko Ishimaru, Kunio Miyake, Takeo Kubota, Hideoki Ogawa, Ko Okumura, Shigenobu Shibata, Atsuhito Nakao

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Background & Aims: The circadian clock drives daily rhythms in behavior and physiology. A recent study suggests that intestinal permeability is also under control of the circadian clock. However, the precise mechanisms remain largely unknown. Because intestinal permeability depends on tight junction (TJ) that regulates the epithelial paracellular pathway, this study investigated whether the circadian clock regulates the expression levels of TJ proteins in the intestine. Methods: The expression levels of TJ proteins in the large intestinal epithelium and colonic permeability were analyzed every 4, 6, or 12 hours between wild-type mice and mice with a mutation of a key clock gene Period2 (Per2; mPer2m/m). In addition, the susceptibility to dextran sodium sulfate (DSS)-induced colitis was compared between wild-type mice and mPer2m/m mice. Results: The mRNA and protein expression levels of Occludin and Claudin-1 exhibited daily variations in the colonic epithelium in wild-type mice, whereas they were constitutively high in mPer2m/m mice. Colonic permeability in wild-type mice exhibited daily variations, which was inversely associated with the expression levels of Occludin and Claudin-1 proteins, whereas it was constitutively low in mPer2m/m mice. mPer2m/m mice were more resistant to the colonic injury induced by DSS than wild-type mice. Conclusions: Occludin and Claudin-1 expressions in the large intestine are under the circadian control, which is associated with temporal regulation of colonic permeability and also susceptibility to colitis.

    Original languageEnglish
    Article numbere98016
    JournalPLoS One
    Volume9
    Issue number5
    DOIs
    Publication statusPublished - 2014 May 20

    Fingerprint

    Claudin-1
    Occludin
    Tight Junction Proteins
    tight junctions
    Large Intestine
    colitis
    large intestine
    Colitis
    Clocks
    Permeability
    permeability
    Dextran Sulfate
    mice
    proteins
    Circadian Clocks
    circadian rhythm
    Physiology
    sodium sulfate
    dextran
    Proteins

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Expressions of tight junction proteins occludin and claudin-1 Are under the circadian control in the mouse large intestine : Implications in intestinal permeability and susceptibility to colitis. / Oh-oka, Kyoko; Kono, Hiroshi; Ishimaru, Kayoko; Miyake, Kunio; Kubota, Takeo; Ogawa, Hideoki; Okumura, Ko; Shibata, Shigenobu; Nakao, Atsuhito.

    In: PLoS One, Vol. 9, No. 5, e98016, 20.05.2014.

    Research output: Contribution to journalArticle

    Oh-oka, Kyoko ; Kono, Hiroshi ; Ishimaru, Kayoko ; Miyake, Kunio ; Kubota, Takeo ; Ogawa, Hideoki ; Okumura, Ko ; Shibata, Shigenobu ; Nakao, Atsuhito. / Expressions of tight junction proteins occludin and claudin-1 Are under the circadian control in the mouse large intestine : Implications in intestinal permeability and susceptibility to colitis. In: PLoS One. 2014 ; Vol. 9, No. 5.
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    AU - Oh-oka, Kyoko

    AU - Kono, Hiroshi

    AU - Ishimaru, Kayoko

    AU - Miyake, Kunio

    AU - Kubota, Takeo

    AU - Ogawa, Hideoki

    AU - Okumura, Ko

    AU - Shibata, Shigenobu

    AU - Nakao, Atsuhito

    PY - 2014/5/20

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