Extended action of MKC-242, a selective 5-HT1A receptor agonist, on light-induced Per gene expression in the suprachiasmatic nucleus in mice

Satomi Takahashi, Yuko Yoshinobu, Reiko Aida, Haruka Shimomura, Masashi Akiyama, Takahiro Moriya, Shigenobu Shibata

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    We reported previously that (S)-5-[3-[(1,4-benzodioxan-2-ylmethyl)amino]propoxy]-1,3-benzodioxole hydrochloride (MKC-242) (3 mg kg-1, i.p.), a selective 5-HT1A receptor agonist, accelerated the re-entrainment of hamster wheel-running rhythms to a new 8 hr delayed or advanced light-dark cycle, and also potentiated the phase advance of the wheel-running rhythm produced by light pulses. The molecular mechanism underlying MKC-242-induced potentiation of this phase shift, however, has not yet been elucidated. We examined the effects of MKC-242 on light-induced mPer1 and mPer2 mRNA expression in the suprachiasmatic nucleus (SCN) of mice. MKC-242 (5 mg kg-1, i.p.) potentiated light-induced mPer1 and mPer2 expression in the SCN of mice housed in constant darkness for 2 days, when mRNA levels were observed 3 hr after light-exposure. More potentiating action of MKC-242 on mPer2 expression in the SCN was observed in mice housed in constant darkness for 9-10 days. This facilitatory action of MKC-242 on mPer1 expression was antagonized by WAY100635, a selective 5-HT1A receptor blocker, indicating that MKC-242 activated 5-HT1A receptors. Other drugs such as 8-hydroxy-dipropylaminotetralin (10 mg kg-1, i.p.), paroxetine (10 mg kg-1, i.p.), buspirone (10 mg kg-1, i.p.), and diazepam (10 mg kg-1, i.p.) did not display a potentiating action on light-induced mPer1 and mPer2 expression in the SCN. In the behavioral experiments, we found that MKC-242 (5 mg kg-1, i.p.) potentiated light-induced phase delays of free-running rhythm in mice. The present results suggest that prolonged increase of mPer1 or mPer2 expression in the SCN by MKC-242 may be involved in the potentiation of photic entrainment by MKC-242 in mice.

    Original languageEnglish
    Pages (from-to)470-478
    Number of pages9
    JournalJournal of Neuroscience Research
    Volume68
    Issue number4
    DOIs
    Publication statusPublished - 2002 May 15

    Fingerprint

    Serotonin 5-HT1 Receptor Agonists
    Suprachiasmatic Nucleus
    Receptor, Serotonin, 5-HT1A
    Gene Expression
    Light
    Running
    Darkness
    osemozotan
    Buspirone
    Paroxetine
    Messenger RNA
    Photoperiod
    Diazepam
    Cricetinae

    Keywords

    • 5-HT receptor
    • Circadian rhythm
    • Light-entrainment
    • MKC-242
    • Per gene
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Extended action of MKC-242, a selective 5-HT1A receptor agonist, on light-induced Per gene expression in the suprachiasmatic nucleus in mice. / Takahashi, Satomi; Yoshinobu, Yuko; Aida, Reiko; Shimomura, Haruka; Akiyama, Masashi; Moriya, Takahiro; Shibata, Shigenobu.

    In: Journal of Neuroscience Research, Vol. 68, No. 4, 15.05.2002, p. 470-478.

    Research output: Contribution to journalArticle

    Takahashi, Satomi ; Yoshinobu, Yuko ; Aida, Reiko ; Shimomura, Haruka ; Akiyama, Masashi ; Moriya, Takahiro ; Shibata, Shigenobu. / Extended action of MKC-242, a selective 5-HT1A receptor agonist, on light-induced Per gene expression in the suprachiasmatic nucleus in mice. In: Journal of Neuroscience Research. 2002 ; Vol. 68, No. 4. pp. 470-478.
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    AU - Yoshinobu, Yuko

    AU - Aida, Reiko

    AU - Shimomura, Haruka

    AU - Akiyama, Masashi

    AU - Moriya, Takahiro

    AU - Shibata, Shigenobu

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