Extracellular production of an RNA aptamer by ribonuclease-free marine bacteria harboring engineered plasmids: A proposal for industrial RNA drug production

Hiromichi Suzuki, Tomoaki Ando, So Umekage, Terumichi Tanaka, Yo Kikuchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Natural noncoding small RNAs have been shown to be involved in a number of cellular processes as regulators. Using the mechanisms thus elucidated, artificial small interfering RNAs (siRNAs), ribozymes, and RNA aptamers are also expected to be potential candidates for RNA therapeutic agents. However, current techniques are too costly for industrial production of these RNAs for use as drugs. Here, we propose a new method for in vivo production of artificial RNAs using the marine phototrophic bacterium Rhodovulum sulfidophilum. Using engineered plasmids and this bacterium, which produces extracellular nucleic acids in nature, we developed a method for extracellular production of a streptavidin RNA aptamer. As the bacterium does not produce any RNases in the culture medium, at least within the cultivation period tested, the designed RNA itself is produced and retained in the culture medium of the bacterium without any specific mechanism for protection against degradation by nucleases. Here, we report that the streptavidin RNA aptamer is produced in the culture medium and retains Its specific function. This Is the first demonstration of extracellular production of a functional artificial RNA in vivo, which will pave the way for inexpensive production of RNA drugs.

Original languageEnglish
Pages (from-to)786-793
Number of pages8
JournalApplied and Environmental Microbiology
Volume76
Issue number3
DOIs
Publication statusPublished - 2010 Feb
Externally publishedYes

Fingerprint

Nucleotide Aptamers
ribonucleases
Ribonucleases
plasmid
RNA
plasmids
drug
Plasmids
Bacteria
drugs
bacterium
bacteria
Pharmaceutical Preparations
Culture Media
Streptavidin
Rhodovulum
streptavidin
culture media
Small Untranslated RNA
Catalytic RNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Extracellular production of an RNA aptamer by ribonuclease-free marine bacteria harboring engineered plasmids : A proposal for industrial RNA drug production. / Suzuki, Hiromichi; Ando, Tomoaki; Umekage, So; Tanaka, Terumichi; Kikuchi, Yo.

In: Applied and Environmental Microbiology, Vol. 76, No. 3, 02.2010, p. 786-793.

Research output: Contribution to journalArticle

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