Extracts of Marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L.

Hitoshi Wake, Hironori Umetsu, Yoshihiro Ozeki, Koichiro Shimomura, Tadashi Matsunaga

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Twenty five strains of marine cyanobacteria were screened for their ability to promote carrot somatic embryogenesis. Hot water extracts prepared from 21 of these strains promoted plantlet formation. Extracts from four strains increased plantlet numbers to an average of over 3.7-fold. Dialysates and nondialysates of each of these extracts also increased plantlet formation. For extracts from filamentous cyanobacteria, Nostoc sp. and Anabaena sp., dialysate was more effective (4.2-fold increase) than nondialysate (3.0-fold increase), whereas for unicellular strains Synechococcus sp. and Xenococcus sp., nondialysate was more effective (5.2-fold increase) than the dialysate (3.2-fold increase). These cyanobacterial extracts also promoted embryolike structure formation from two-year old carrot cell cultures which were unable to produce plantlets using the usual methods. Here, we demonstrate the existence in marine cyanobacterial extracts of low and high molecular weight factors which strongly promote somatic embryogenesis in carrot cell cultures.

Original languageEnglish
Pages (from-to)655-658
Number of pages4
JournalPlant Cell Reports
Volume9
Issue number12
DOIs
Publication statusPublished - 1991 Apr
Externally publishedYes

Fingerprint

Daucus carota
somatic embryogenesis
Cyanobacteria
plantlets
extracts
carrots
Xenococcus
cell culture
Nostoc
Anabaena
Synechococcus
molecular weight
water

Keywords

  • Cyanobacteria
  • Daucus carota L
  • Plant Growth Regulator
  • Plant Regeneration

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Extracts of Marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L. / Wake, Hitoshi; Umetsu, Hironori; Ozeki, Yoshihiro; Shimomura, Koichiro; Matsunaga, Tadashi.

In: Plant Cell Reports, Vol. 9, No. 12, 04.1991, p. 655-658.

Research output: Contribution to journalArticle

Wake, H, Umetsu, H, Ozeki, Y, Shimomura, K & Matsunaga, T 1991, 'Extracts of Marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L.', Plant Cell Reports, vol. 9, no. 12, pp. 655-658. https://doi.org/10.1007/BF00235350
Wake, Hitoshi ; Umetsu, Hironori ; Ozeki, Yoshihiro ; Shimomura, Koichiro ; Matsunaga, Tadashi. / Extracts of Marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L. In: Plant Cell Reports. 1991 ; Vol. 9, No. 12. pp. 655-658.
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