Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Ayako Oyane, Yushin Yazaki, Hiroko Araki, Yu Sogo, Atsuo Ito, Atsushi Yamazaki, Hideo Tsurushima

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    A surface-mediated gene transfer system using biocompatible apatite-based composite layers has great potential for tissue engineering. Among the apatite-based composite layers developed to date, we focused on a DNAlipid- apatite composite layer (DLp-Ap layer), which has the advantage of relatively high efficiency as a non-viral system. In this study, various lipid transfection reagents, including a newly developed reagent, polyamidoamine dendron-bearing lipid (PD), were employed to prepare the DLp-Ap layer, and the preparation condition was optimized in terms of efficiency of gene transfer to epithelial-like CHO-K1 cells in the presence of serum. The optimized DLp-Ap layer derived from PD had the highest gene transfer efficiency among all the apatite-based composite layers prepared in this study. In addition, the optimized DLp-Ap layer demonstrated higher gene transfer efficiency in the presence of serum than the conventional particle-mediated systems using commercially available lipid transfection reagents. It was also shown that the optimized DLp-Ap layer mediated the area-specific gene transfer on its surface, i.e., DNA was preferentially transferred to the cells adhering to the surface of the layer. The present gene transfer system using the PD-derived DLp-Ap layer, with the advantages of high efficiency in the presence of serum and area-specificity, would be useful in tissue engineering.

    Original languageEnglish
    Pages (from-to)1011-1019
    Number of pages9
    JournalJournal of Materials Science: Materials in Medicine
    Volume23
    Issue number4
    DOIs
    Publication statusPublished - 2012 Apr

    Fingerprint

    Gene transfer
    Apatites
    Apatite
    Lipids
    DNA
    Fabrication
    Composite materials
    Bearings (structural)
    Genes
    Tissue Engineering
    Tissue engineering
    Transfection
    Serum
    CHO Cells
    Bioelectric potentials

    ASJC Scopus subject areas

    • Biophysics
    • Biomaterials
    • Bioengineering
    • Biomedical Engineering

    Cite this

    Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer. / Oyane, Ayako; Yazaki, Yushin; Araki, Hiroko; Sogo, Yu; Ito, Atsuo; Yamazaki, Atsushi; Tsurushima, Hideo.

    In: Journal of Materials Science: Materials in Medicine, Vol. 23, No. 4, 04.2012, p. 1011-1019.

    Research output: Contribution to journalArticle

    Oyane, Ayako ; Yazaki, Yushin ; Araki, Hiroko ; Sogo, Yu ; Ito, Atsuo ; Yamazaki, Atsushi ; Tsurushima, Hideo. / Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer. In: Journal of Materials Science: Materials in Medicine. 2012 ; Vol. 23, No. 4. pp. 1011-1019.
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