Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer

Ayako Oyane, Yushin Yazaki, Hiroko Araki, Yu Sogo, Atsuo Ito, Atsushi Yamazaki, Hideo Tsurushima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A surface-mediated gene transfer system using biocompatible apatite-based composite layers has great potential for tissue engineering. Among the apatite-based composite layers developed to date, we focused on a DNAlipid- apatite composite layer (DLp-Ap layer), which has the advantage of relatively high efficiency as a non-viral system. In this study, various lipid transfection reagents, including a newly developed reagent, polyamidoamine dendron-bearing lipid (PD), were employed to prepare the DLp-Ap layer, and the preparation condition was optimized in terms of efficiency of gene transfer to epithelial-like CHO-K1 cells in the presence of serum. The optimized DLp-Ap layer derived from PD had the highest gene transfer efficiency among all the apatite-based composite layers prepared in this study. In addition, the optimized DLp-Ap layer demonstrated higher gene transfer efficiency in the presence of serum than the conventional particle-mediated systems using commercially available lipid transfection reagents. It was also shown that the optimized DLp-Ap layer mediated the area-specific gene transfer on its surface, i.e., DNA was preferentially transferred to the cells adhering to the surface of the layer. The present gene transfer system using the PD-derived DLp-Ap layer, with the advantages of high efficiency in the presence of serum and area-specificity, would be useful in tissue engineering.

Original languageEnglish
Pages (from-to)1011-1019
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume23
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer'. Together they form a unique fingerprint.

Cite this