Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase

Takashi Tanii, T. Goto, T. Iida, M. Koh-Masahara, I. Ohdomari

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.

    Original languageEnglish
    JournalJapanese Journal of Applied Physics, Part 2: Letters
    Volume40
    Issue number10 B
    Publication statusPublished - 2001 Oct 15

    Fingerprint

    adenosine triphosphate
    enzymes
    Enzymes
    chips
    Photodiodes
    Fabrication
    fabrication
    Molecules
    molecules
    Silicon
    photodiodes
    silicon
    proportion
    plots

    Keywords

    • ATP
    • Luciferase
    • Luciferin
    • Photodiode transducer
    • Silicon

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

    Cite this

    Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase. / Tanii, Takashi; Goto, T.; Iida, T.; Koh-Masahara, M.; Ohdomari, I.

    In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 40, No. 10 B, 15.10.2001.

    Research output: Contribution to journalArticle

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