Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase

T. Tanii, T. Goto, T. Iida, M. Koh-Masahara, I. Ohdomari

Research output: Contribution to journalLetterpeer-review

21 Citations (Scopus)

Abstract

We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.

Original languageEnglish
Pages (from-to)L1135-L1137
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume40
Issue number10 B
DOIs
Publication statusPublished - 2001 Oct 15

Keywords

  • ATP
  • Luciferase
  • Luciferin
  • Photodiode transducer
  • Silicon

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy (miscellaneous)
  • Physics and Astronomy(all)

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