Abstract
We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.
| Original language | English |
|---|---|
| Journal | Japanese Journal of Applied Physics, Part 2: Letters |
| Volume | 40 |
| Issue number | 10 B |
| Publication status | Published - 2001 Oct 15 |
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Keywords
- ATP
- Luciferase
- Luciferin
- Photodiode transducer
- Silicon
ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase. / Tanii, Takashi; Goto, T.; Iida, T.; Koh-Masahara, M.; Ohdomari, I.
In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 40, No. 10 B, 15.10.2001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fabrication of adenosine triphosphate-molecule recognition chip by means of bioluminous enzyme luciferase
AU - Tanii, Takashi
AU - Goto, T.
AU - Iida, T.
AU - Koh-Masahara, M.
AU - Ohdomari, I.
PY - 2001/10/15
Y1 - 2001/10/15
N2 - We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.
AB - We have succeeded in detecting the adenosine triphosphate (ATP) concentration in a solution quantitatively using an ATP-molecule recognition chip. The ATP-molecule recognition chip is composed of a silicon photodiode on which bioluminous enzyme luciferase is immobilized. When the chip was immersed in an ATP-containing solution, the luciferase emitted light and the photoinduced current detected by the photodiode was in proportion to the ATP concentration. We found that the photoinduced current fits the Michaelis-Menten plot. These results indicate that the luciferase is successfully immobilized on the silicon chip without losing the bioluminous activity and that the proposed device enables us to detect the ATP concentration in a solution by measuring the photoinduced current.
KW - ATP
KW - Luciferase
KW - Luciferin
KW - Photodiode transducer
KW - Silicon
UR - http://www.scopus.com/inward/record.url?scp=0035888743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035888743&partnerID=8YFLogxK
M3 - Article
VL - 40
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 10 B
ER -