Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer

Yushin Yazaki, Ayako Oyane, Hiroko Araki, Yu Sogo, Atsuo Ito, Atsushi Yamazaki, Hideo Tsurushima

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Surface-mediated gene transfer systems using apatite (Ap)-based composite layers have received increased attention in tissue engineering applications owing to their safety, biocompatibility and relatively high efficiency. In this study, DNA-antibody-apatite composite layers (DA-Ap layers), in which DNA and antibody molecules are immobilized within a matrix of apatite nanocrystals, were fabricated using a biomimetic coating process. They were then assayed for their gene transfer capability for application in a specific cell-targeted gene transfer. A DA-Ap layer that was fabricated with an anti-CD49f antibody showed a higher gene transfer capability to the CD49f-positive CHO-K1 cells than a DNA-apatite composite layer (D-Ap layer). The antibody facilitated the gene transfer capability of the DA-Ap layer only to the specific cells that were expressing corresponding antigens. When the DA-Ap layer was fabricated with an anti-N-cadherin antibody, a higher gene transfer capability compared with the D-Ap layer was found in the N-cadherin-positive P19CL6 cells, but not in the N-cadherin-negative UV♀2 cells or in the P19CL6 cells that were pre-blocked with anti-N-cadherin. Therefore, the antigen-antibody binding that takes place at the cell-layer interface should be responsible for the higher gene transfer capability of the DA-Ap than D-Ap layer. These results suggest that the DA-Ap layer works as a mediator in a specific cell-targeted gene transfer system.

    Original languageEnglish
    Article number064204
    JournalScience and Technology of Advanced Materials
    Volume13
    Issue number6
    DOIs
    Publication statusPublished - 2012 Dec

    Fingerprint

    Gene transfer
    Apatites
    Apatite
    Antibodies
    DNA
    Fabrication
    Composite materials
    Cadherins
    Antigens
    Biomimetics
    Biocompatibility
    Tissue engineering
    Nanocrystals

    Keywords

    • antibody
    • cell targeting
    • coating
    • composite
    • hydroxyapatite
    • transfection

    ASJC Scopus subject areas

    • Materials Science(all)

    Cite this

    Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer. / Yazaki, Yushin; Oyane, Ayako; Araki, Hiroko; Sogo, Yu; Ito, Atsuo; Yamazaki, Atsushi; Tsurushima, Hideo.

    In: Science and Technology of Advanced Materials, Vol. 13, No. 6, 064204, 12.2012.

    Research output: Contribution to journalArticle

    Yazaki, Yushin ; Oyane, Ayako ; Araki, Hiroko ; Sogo, Yu ; Ito, Atsuo ; Yamazaki, Atsushi ; Tsurushima, Hideo. / Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer. In: Science and Technology of Advanced Materials. 2012 ; Vol. 13, No. 6.
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