Fabrication of electrodes for highly sensitive detection of a superoxide anion radical by electropolymerization of thienylporphyrins in the presence of thiophene and application to active oxygen sensors

Makoto Yuasa, Kenichi Oyaizu, Hidenori Murata, Masuhide Ishikawa, Satoshi Tsutsui, Masahiro Namba

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The superoxide anion radical (O2 -·) has attracted much attention in many fields of biology since it was realized that superoxide anions are produced in significant quantities in various parts of biological systems, from microorganisms to mammals. Therefore, quantitative measurements of O2 -· in vivo are important for clarifying such relationships under various conditions. However, in most cases, the biological significance and the mechanism of the generation O2 -· are not yet well understood. We have developed an O2 - · sensor composed of a thin film of 1-methylimidazole-coordinated iron meso-tetra(3-thienyl)porphyrin ([Fe(im)2(ttp)]Br) for the electrochemical detection of O2 -· The microsensor displayed high selectivity and activity for the oxidation of O2 -· and showed a linear relationship between the current and the O2 -· concentration. In this research, the improvement of the sensor sensitivity was accomplished by mixing polythiophene as the electroconductive polymer on the electrode surface. The sensors were prepared using various compositions of [Fe(im)2(ttp)]Br and thiophene as the monomers. During in vitro tests, O2 -· was generated as an intermediate during the oxidation of xanthine by xanthine oxidase (XOD), for detection using this sensor. The electric conductivity of the polymer increased, accompanied by the increase in the amount of the thiophene unit. The highest sensitivity was obtained at a monomer composition of [Fe(im)2(ttp)]Br : thiophene = 1:8.

Original languageEnglish
Pages (from-to)427-431
Number of pages5
JournalKobunshi Ronbunshu
Volume63
Issue number6
DOIs
Publication statusPublished - 2006 Jun
Externally publishedYes

Fingerprint

Thiophenes
Oxygen sensors
Electropolymerization
Thiophene
Superoxides
anion
Reactive Oxygen Species
electrode
Negative ions
sensor
Fabrication
oxygen
Electrodes
Sensors
Polymers
polymer
Monomers
oxidation
Oxidation
Microsensors

Keywords

  • Electrocatalyst
  • Electropolymerization
  • Porphyrin complex
  • Sensor
  • Superoxide anion radical

ASJC Scopus subject areas

  • Polymers and Plastics
  • Environmental Science(all)
  • Materials Science (miscellaneous)
  • Chemical Engineering (miscellaneous)
  • Ceramics and Composites

Cite this

Fabrication of electrodes for highly sensitive detection of a superoxide anion radical by electropolymerization of thienylporphyrins in the presence of thiophene and application to active oxygen sensors. / Yuasa, Makoto; Oyaizu, Kenichi; Murata, Hidenori; Ishikawa, Masuhide; Tsutsui, Satoshi; Namba, Masahiro.

In: Kobunshi Ronbunshu, Vol. 63, No. 6, 06.2006, p. 427-431.

Research output: Contribution to journalArticle

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abstract = "The superoxide anion radical (O2 -·) has attracted much attention in many fields of biology since it was realized that superoxide anions are produced in significant quantities in various parts of biological systems, from microorganisms to mammals. Therefore, quantitative measurements of O2 -· in vivo are important for clarifying such relationships under various conditions. However, in most cases, the biological significance and the mechanism of the generation O2 -· are not yet well understood. We have developed an O2 - · sensor composed of a thin film of 1-methylimidazole-coordinated iron meso-tetra(3-thienyl)porphyrin ([Fe(im)2(ttp)]Br) for the electrochemical detection of O2 -· The microsensor displayed high selectivity and activity for the oxidation of O2 -· and showed a linear relationship between the current and the O2 -· concentration. In this research, the improvement of the sensor sensitivity was accomplished by mixing polythiophene as the electroconductive polymer on the electrode surface. The sensors were prepared using various compositions of [Fe(im)2(ttp)]Br and thiophene as the monomers. During in vitro tests, O2 -· was generated as an intermediate during the oxidation of xanthine by xanthine oxidase (XOD), for detection using this sensor. The electric conductivity of the polymer increased, accompanied by the increase in the amount of the thiophene unit. The highest sensitivity was obtained at a monomer composition of [Fe(im)2(ttp)]Br : thiophene = 1:8.",
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